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EC 1.14.13.201 Details
show Brenda entryEC number
1.14.13.201
Accepted name
β-amyrin 28-monooxygenase
Reaction
β-amyrin + 3 O2 + 3 NADPH + 3 H+ = oleanolate + 3 NADP+ + 4 H2O (overall reaction);;(1a) β-amyrin + O2 + NADPH + H+ = erythrodiol + NADP+ + H2O;;(1b) erythrodiol + O2 + NADPH + H+ = oleanolic aldehyde + NADP+ + 2 H2O;;(1c) oleanolic aldehyde + O2 + NADPH + H+ = oleanolate + NADP+ + H2O
Other name(s)
CYP716A52v2,
CYP716A12,
CYP16A75,
β-amyrin 28-oxidase
Systematic name
β-amyrin,NADPH:oxygen oxidoreductase (28-hydroxylating)
Comment
The enzyme, found in plants, is involved in the biosynthesis of oleanane-type triterpenoids, such as ginsenoside Ro. The enzyme from Medicago truncatula (barrel medic) (CYP716A12) can also convert α-amyrin and lupeol to ursolic acid and betulinic acid, respectively. The enzyme from Maesa lanceolata (false assegai) (CYP16A75) does not catalyse the reaction to completion, resulting in accumulation of both intermediates.
History
created 2015, deleted 2018
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1 Oxidoreductases
1.1 Acting on the CH-OH group of donors
1.14.13.45 created 1992, deleted 2003
