Any feedback?
Please rate this page
(ecexplorer.php)
(0/150)

BRENDA support

EC Explorer

EC 1.14.13.39 Details
EC number
1.14.13.39
Accepted name
nitric-oxide synthase (NADPH)
Reaction
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O (overall reaction);;(1a) 2 L-arginine + 2 NADPH + 2 H+ + 2 O2 = 2 Nω-hydroxy-L-arginine + 2 NADP+ + 2 H2O ;;(1b) 2 Nω-hydroxy-L-arginine + NADPH + H+ + 2 O2 = 2 L-citrulline + 2 nitric oxide + NADP+ + 2 H2O
Other name(s)
NOS (gene name), nitric oxide synthetase (ambiguous), endothelium-derived relaxation factor-forming enzyme, endothelium-derived relaxing factor synthase, NO synthase (ambiguous), NADPH-diaphorase (ambiguous)
Systematic name
L-arginine,NADPH:oxygen oxidoreductase (nitric-oxide-forming)
CAS registry number
125978-95-2
Comment
The enzyme consists of linked oxygenase and reductase domains. The eukaryotic enzyme binds FAD, FMN, heme (iron protoporphyrin IX) and tetrahydrobiopterin, and its two domains are linked via a regulatory calmodulin-binding domain. Upon calcium-induced calmodulin binding, the reductase and oxygenase domains form a complex, allowing electrons to flow from NADPH via FAD and FMN to the active center. The reductase domain of the enzyme from the bacterium Sorangium cellulosum utilizes a [2Fe-2S] cluster to transfer the electrons from NADPH to the active center. cf. EC 1.14.14.47, nitric-oxide synthase (flavodoxin).
History
created 1992, modified 2012, modified 2017
EC Tree
1.14.13.45 created 1992, deleted 2003