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EC 1.14.13.247 Details
show Brenda entryEC number
1.14.13.247
Accepted name
stachydrine N-demethylase
Reaction
L-proline betaine + NAD(P)H + H+ + O2 = N-methyl-L-proline + formaldehyde + NAD(P)+ + H2O
Other name(s)
L-proline betaine N-demethylase,
stc2 (gene name)
Systematic name
L-proline betaine,NAD(P)H:oxygen oxidoreductase (formaldehyde-forming)
Comment
The enzyme, characterized from the bacterium Sinorhizobium meliloti 1021, consists of three different types of subunits. The catalytic unit contains a Rieske [2Fe-2S] iron-sulfur cluster, and catalyses the monooxygenation of a methyl group. The resulting N-methoxyl group is unstable and decomposes spontaneously to form formaldehyde. The other subunits are involved in the transfer of electrons from NAD(P)H to the catalytic subunit.
History
created 2017
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1 Oxidoreductases
1.1 Acting on the CH-OH group of donors
1.14.13.45 created 1992, deleted 2003
