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EC 1.14.13.243 Details
show Brenda entryEC number
1.14.13.243
Accepted name
toluene 2-monooxygenase
Reaction
(1) toluene + NADH + H+ + O2 = 2-methylphenol + NAD+ + H2O;;(2) 2-methylphenol + NADH + H+ + O2 = 3-methylcatechol + NAD+ + H2O
Other name(s)
tomA1/2/3/4/5 (gene names),
toluene ortho-monooxygenase
Systematic name
toluene,NADH:oxygen oxidoreductase (2,3-dihydroxylating)
Comment
The enzyme, characterized from the bacterium Burkholderia cepacia, belongs to a class of nonheme, oxygen-dependent diiron enzymes. It contains a hydroxylase component with two binuclear iron centers, an NADH-oxidoreductase component containing FAD and a [2Fe-2S] iron-sulfur cluster, and a third component involved in electron transfer between the hydroxylase and the reductase. The enzyme dihydroxylates its substrate in two sequential hydroxylations, initially forming 2-methylphenol, which is hydroxylated to 3-methylcatechol.
History
created 2019
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1 Oxidoreductases
1.1 Acting on the CH-OH group of donors
1.14.13.45 created 1992, deleted 2003
