EC Explorer
[browse EC tree] - [search]
EC 1.14.13.142 Details
show Brenda entryEC number
1.14.13.142
Accepted name
3-ketosteroid 9α-monooxygenase
Reaction
androsta-1,4-diene-3,17-dione + NADH + H+ + O2 = 9α-hydroxyandrosta-1,4-diene-3,17-dione + NAD+ + H2O
Other name(s)
KshAB,
3-ketosteroid 9α-hydroxylase
Systematic name
androsta-1,4-diene-3,17-dione,NADH:oxygen oxidoreductase (9α-hydroxylating)
Comment
The enzyme is involved in the cholesterol degradation pathway of several bacterial pathogens, such as Mycobacterium tuberculosis. It is a two-component system consisting of a terminal oxygenase (KshA) and a ferredoxin reductase (KshB). The oxygenase contains a Rieske-type iron-sulfur center and non-heme iron. The reductase component is a flavoprotein containing an NAD-binding domain and a plant-type iron-sulfur cluster. The product of the enzyme is unstable, and spontaneously converts to 3-hydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione.
History
created 2012, deleted 2018
back to topEC Tree
1 Oxidoreductases
1.1 Acting on the CH-OH group of donors
1.14.13.45 created 1992, deleted 2003
