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EC 1.14.13.187 Details
show Brenda entryEC number
1.14.13.187
Accepted name
L-evernosamine nitrososynthase
Reaction
dTDP-β-L-evernosamine + 2 NADPH + 2 H+ + 2 O2 = dTDP-2,3,6-trideoxy-3-C-methyl-4-O-methyl-3-nitroso-β-L-arabino-hexopyranose + 2 NADP+ + 3 H2O (overall reaction);;(1a) dTDP-β-L-evernosamine + NADPH + H+ + O2 = dTDP-N-hydroxy-β-L-evernosamine + NADP+ + H2O;;(1b) dTDP-N-hydroxy-β-L-evernosamine + NADPH + H+ + O2 = dTDP-2,3,6-trideoxy-3-C-methyl-4-O-methyl-3-nitroso-β-L-arabino-hexopyranose + NADP+ + 2 H2O
Systematic name
dTDP-β-L-evernosamine,NADPH:oxygen oxidoreductase (N-hydroxylating)
Comment
Requires FAD. Isolated from the bacterium Micromonospora carbonacea var. africana. The nitroso group is probably spontaneously oxidized to a nitro group giving dTDP-β-L-evernitrose, which is involved in the biosynthesis of the antibiotic everninomycin. The reaction was studied using dTDP-β-L-4-epi-vancosamine (dTDP-4-O-desmethyl-β-L-evernitrosamine).
History
created 2014
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1 Oxidoreductases
1.1 Acting on the CH-OH group of donors
1.14.13.45 created 1992, deleted 2003
