1.14.14.87: 2-hydroxyisoflavanone synthase
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For detailed information about 2-hydroxyisoflavanone synthase, go to the full flat file.
Word Map on EC 1.14.14.87
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1.14.14.87
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soybean
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chalcone
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isoflavonoids
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genistein
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daidzein
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formononetin
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soja
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glyceollin
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non-legume
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medicarpin
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legume-specific
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synthesis
- 1.14.14.87
- soybean
- chalcone
-
isoflavonoids
- genistein
- daidzein
- formononetin
-
soja
- glyceollin
-
non-legume
- medicarpin
-
legume-specific
- synthesis
Reaction
Synonyms
2-HIS, 2-hydroxyisoflavanone synthase, 2-hydroxyisoflavanone synthase 1, 2HI synthase, CYP93C, Cyp93C1, CYP93C18, CYP93C2, Cyp93C20, CYT93C, EC 1.14.13.136, EC 1.14.13.86, GmIFS, GmIFS1, IFS, IFS1, IFS2, IFS2-12, isoflavone synthase, isoflavone synthase 2
ECTree
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General Information on EC 1.14.14.87 - 2-hydroxyisoflavanone synthase
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GENERAL INFORMATION 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
malfunction
overexpression and RNAi-mediated silencing of GmMYB29 in soybean hairy roots resulted in increased and decreased isoflavone content, respectively. 11 Natural GmMYB29 polymorphisms are significantly associated with isoflavone contents, and regulation of GmMYB29 expression partially contributes to the observed phenotypic variation, the detected single nucleotide polymorphism is SNP AX-93910416, detected within the 5'-untranslated region of Glyma20g35180 (GmMYB29)
metabolism
physiological function
metabolism
(2S)-flavanones undergo 2-hydroxylation catalyzed by 2-hydroxyisoflavanone synthase (GmIFS), a microsomal cytochrome P450 enzyme (CYP93C), to produce 2-hydroxyisoflavanones pathway of isoflavone biosynthesis in soybean. Analysis of binary protein-protein interactions of 2-hydroxyisoflavanone synthase 1 (GmIFS1), a P450 (CYP93C), with cytoplasmic enzymes involved in isoflavone biosynthesis in soybean, by split-ubiquitin system. Identification of binary interactions between GmIFS1 and chalcone synthase 1 (GmCHS1), and between GmIFS1 and chalcone isomerases (GmCHIs), the interaction of GmCHS1 and GmCHIs with GmIFS1 takes place on endoplasmic reticulum on which GmIFS1 is located
metabolism
the critical branch point enzymes chalcone synthase (CHS) and isoflavone synthase (IFS) lead substrates to the isoflavone synthesis branch and finally generate isoflavones and their derivatives, isoflavone regulation-related transcription factors, overview. Positive regulatory role of GmMYB29 in isoflavone biosynthesis
physiological function
both transient and stable expression of the IFS2_12 or IFS/alfalfa chalcone isomerase constructs in Nicotiana tabacum result in the production of the isoflavonoid genistein and its conjugates
physiological function
constitutive expression of isoflavone synthase in alfalfa (Medicago sativa) leaves leads to genistein glucoside production (up to 50 nmol/g fresh weight) and to accumulation of glucosides of biochanin A and pratensein. IFS1 is highly expressed in all transgenic organs examined, while genistein accumulation is limited to leaves. IFS1-expressing lines accumulate additional isoflavones, including formononetin and daidzein and the phytoalexin medicarpin, in response to UV-B or Phoma medicaginis. IFS1 expression does not significantly alter global gene expression in the leaves
physiological function
enzyme shows binary interactions with cytoplasmic chalcone synthase 1 and chalcone isomerases CHI, both in recombinant Saccharomyces cerevisiae and in planta
physiological function
expression in Arabidopsis thaliana leads to production of the isoflavone genistein
physiological function
expression in Brassica napus plants via Agrobacterium tumefaciens-mediated transformation direct the synthesis and accumulation of genistein derivatives in leaves up to 0.72 mg/g dry weight. In addition, expression levels for most Brassica napus genes in the phenylpropanoid pathway are altered
physiological function
the expression of the IFS gene in Oryza sativa leads to the production of the isoflavone genistein in rice tissues. The genistein produced in rice cells is present in a glycoside form. The expression of isoflavone synthase confers rice plants with the ability to produce flavonoids that are able to induce nod gene expression, albeit to varied degrees, in different rhizobia
physiological function
upon coexpression of IFS with rice P450 reductase in yeast, the production of genistein from naringein increases about 4.3fold
