1.14.14.87: 2-hydroxyisoflavanone synthase

This is an abbreviated version!
For detailed information about 2-hydroxyisoflavanone synthase, go to the full flat file.

Reaction

Synonyms

2-HIS, 2-hydroxyisoflavanone synthase, 2-hydroxyisoflavanone synthase 1, 2HI synthase, CYP93C, Cyp93C1, CYP93C18, CYP93C2, Cyp93C20, CYT93C, EC 1.14.13.136, EC 1.14.13.86, GmIFS, GmIFS1, IFS, IFS1, IFS2, IFS2-12, isoflavone synthase, isoflavone synthase 2

ECTree

     1 Oxidoreductases

         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor

                1.14.14.87 2-hydroxyisoflavanone synthase

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Results	in table
1	Activating Compound
19	AA Sequence
1	Application
9	Cloned(Commentary)
6	Cofactor
3	Expression
10	General Information
5	Inhibitors
4	KM Value [mM]
8	Localization
1	Metals/Ions
1	Molecular Weight [Da]
6	Natural Substrates/ Products (Substrates)
22	Organism
5	Pathway
2	pH Optimum
2	pH Range
9	Protein Variants
2	Reaction
21	Reference
7	Source Tissue
20	Substrates and Products (Substrate)
2	Subunits
37	Synonyms
1	Systematic Name

Application

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Application on EC 1.14.14.87 - 2-hydroxyisoflavanone synthase

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APPLICATION

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

synthesis

Trifolium pratense

Q84QI4

construction of an in-frame fusion of IFS and cytochrome P450 reductase from rice after removing the membrane binding domain of both enzymes. Expression of the fusion construct in Escherichia coli leads to conversion of naringenin into genistein. Upon optimization of conditions, 60 microM of genistein can be generated from 80 microM of naringenin

745537