1.14.14.87: 2-hydroxyisoflavanone synthase
This is an abbreviated version!
For detailed information about 2-hydroxyisoflavanone synthase, go to the full flat file.
Word Map on EC 1.14.14.87
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1.14.14.87
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soybean
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chalcone
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isoflavonoids
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genistein
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daidzein
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formononetin
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soja
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glyceollin
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non-legume
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medicarpin
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legume-specific
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synthesis
- 1.14.14.87
- soybean
- chalcone
-
isoflavonoids
- genistein
- daidzein
- formononetin
-
soja
- glyceollin
-
non-legume
- medicarpin
-
legume-specific
- synthesis
Reaction
Synonyms
2-HIS, 2-hydroxyisoflavanone synthase, 2-hydroxyisoflavanone synthase 1, 2HI synthase, CYP93C, Cyp93C1, CYP93C18, CYP93C2, Cyp93C20, CYT93C, EC 1.14.13.136, EC 1.14.13.86, GmIFS, GmIFS1, IFS, IFS1, IFS2, IFS2-12, isoflavone synthase, isoflavone synthase 2
ECTree
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Engineering
Engineering on EC 1.14.14.87 - 2-hydroxyisoflavanone synthase
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K375T
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the mutant catalyses the aryl migration from liquiritigenin to yield 3,7,4'-trihydroxyflavanone only, its pH optimum is at pH 6.5
L371V/K375T
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the mutant shows 3-5% activity levels compared to the wild type. The mutant produces a mixture of 95% 3beta-hydroxyflavanone and 5% flavone from (2S)-liquiritigenin
S310T
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the mutant also catalyses the aryl migration from liquiritigenin to yield 2,7,4'-trihydroxyisoflavanone, but the ratio of the by-product (3,7,4'-trihydroxyflavanone) formation is increased from 8% to 36% (pH 7.5), and a small amount (7%) of a new product, 7,4'-dihydroxyflavone, is detected compared to the wild type
S310T/K375T
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the P450 level of the mutant is approximately 1.5times that of wild type. The mutant shows 3-5% activity levels compared to the wild type
S310T/L371V/K375T
additional information
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the mutant produces 100% flavone from (2S)-liquiritigenin
S310T/L371V/K375T
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the mutant shows 4times higher P450 level than the wild type
oestrogen-deficient (ovariectomized, Ovx) mice fed with leaf extract from transgenic plant co-expressing AtMYB12 and GmIFS1 but not wild-type extract exhibit significant conservation of trabecular microarchitecture, reduced osteoclast number and expression of osteoclastogenic genes, higher total serum antioxidant levels, and increased uterine oestrogenicity compared with Ovx mice treated with vehicle (control). The simultaneous coexpression of AtMYB12 and GmIFS1 in tobacco leads to the accumulation of substantial amount of genistein in addition to flavonols
additional information
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oestrogen-deficient (ovariectomized, Ovx) mice fed with leaf extract from transgenic plant co-expressing AtMYB12 and GmIFS1 but not wild-type extract exhibit significant conservation of trabecular microarchitecture, reduced osteoclast number and expression of osteoclastogenic genes, higher total serum antioxidant levels, and increased uterine oestrogenicity compared with Ovx mice treated with vehicle (control). The simultaneous coexpression of AtMYB12 and GmIFS1 in tobacco leads to the accumulation of substantial amount of genistein in addition to flavonols
additional information
deletion of the membrane-spanning domain and insertion of a hydrophilic polypeptide in the N-terminus and a four histidine tag at the C-terminus results dramatically enhanced expression in Escherichia coli and and solubility. The purified enzyme exhibits the same activity as the full length membrane-associated enzyme expressed in yeast
additional information
-
deletion of the membrane-spanning domain and insertion of a hydrophilic polypeptide in the N-terminus and a four histidine tag at the C-terminus results dramatically enhanced expression in Escherichia coli and and solubility. The purified enzyme exhibits the same activity as the full length membrane-associated enzyme expressed in yeast