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1.14.14.87: 2-hydroxyisoflavanone synthase

This is an abbreviated version!
For detailed information about 2-hydroxyisoflavanone synthase, go to the full flat file.

Word Map on EC 1.14.14.87

Reaction

(2S)-naringenin
+
O2
+
[reduced NADPH-hemoprotein reductase]
=
2,4',5,7-tetrahydroxyisoflavanone
+
H2O
+
[oxidized NADPH-hemoprotein reductase]

Synonyms

2-HIS, 2-hydroxyisoflavanone synthase, 2-hydroxyisoflavanone synthase 1, 2HI synthase, CYP93C, Cyp93C1, CYP93C18, CYP93C2, Cyp93C20, CYT93C, EC 1.14.13.136, EC 1.14.13.86, GmIFS, GmIFS1, IFS, IFS1, IFS2, IFS2-12, isoflavone synthase, isoflavone synthase 2

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.14.87 2-hydroxyisoflavanone synthase

Cloned

Cloned on EC 1.14.14.87 - 2-hydroxyisoflavanone synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Arabidopsis thaliana and in leaves of Nicotiana benthamiana
expressed in Saccharomyces cerevisiae microsomes
-
expressed in Saccharomyces cerevisiae strain BJ2168
-
expression in insect cells
expression in Saccharomyces cerevisiae
gene ifs1, full-length GmIFS1 cDNA is isolated through RT-PCR of total RNA of soybean seedling, recombinant coexpression of Arabidopsis thaliana transcription factor, AtMYB12, and Glycine max isoflavone synthase, GmIFS1, genes in Nicotiana tabacum cv. Petit Havana via Agrobacterium tumefaciens strain LBA4404 transformation method leading to enhanced biosynthesis of isoflavones and flavonols resulting in osteoprotective activity. real-time PCR and RT-PCR enzyme expression analysis
gene ifs1, protein-protein interaction analysis using the split-ubiquitin system, in planta by BiFC, and the yeast two-hybrid system in Saccharomyces cerevisiae. No appreciable yeast growth is detected with any combinations of proteins under the assay conditions