1.14.14.87: 2-hydroxyisoflavanone synthase
This is an abbreviated version!
For detailed information about 2-hydroxyisoflavanone synthase, go to the full flat file.
Word Map on EC 1.14.14.87
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1.14.14.87
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soybean
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chalcone
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isoflavonoids
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genistein
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daidzein
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formononetin
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soja
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glyceollin
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non-legume
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medicarpin
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legume-specific
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synthesis
- 1.14.14.87
- soybean
- chalcone
-
isoflavonoids
- genistein
- daidzein
- formononetin
-
soja
- glyceollin
-
non-legume
- medicarpin
-
legume-specific
- synthesis
Reaction
Synonyms
2-HIS, 2-hydroxyisoflavanone synthase, 2-hydroxyisoflavanone synthase 1, 2HI synthase, CYP93C, Cyp93C1, CYP93C18, CYP93C2, Cyp93C20, CYT93C, EC 1.14.13.136, EC 1.14.13.86, GmIFS, GmIFS1, IFS, IFS1, IFS2, IFS2-12, isoflavone synthase, isoflavone synthase 2
ECTree
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Cloned
Cloned on EC 1.14.14.87 - 2-hydroxyisoflavanone synthase
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expressed in Arabidopsis thaliana and in leaves of Nicotiana benthamiana
expression in Saccharomyces cerevisiae
gene ifs1, full-length GmIFS1 cDNA is isolated through RT-PCR of total RNA of soybean seedling, recombinant coexpression of Arabidopsis thaliana transcription factor, AtMYB12, and Glycine max isoflavone synthase, GmIFS1, genes in Nicotiana tabacum cv. Petit Havana via Agrobacterium tumefaciens strain LBA4404 transformation method leading to enhanced biosynthesis of isoflavones and flavonols resulting in osteoprotective activity. real-time PCR and RT-PCR enzyme expression analysis
gene ifs1, protein-protein interaction analysis using the split-ubiquitin system, in planta by BiFC, and the yeast two-hybrid system in Saccharomyces cerevisiae. No appreciable yeast growth is detected with any combinations of proteins under the assay conditions