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2.3.1.B43: protein-lysine desuccinylase (NAD+)

This is an abbreviated version!
For detailed information about protein-lysine desuccinylase (NAD+), go to the full flat file.

Word Map on EC 2.3.1.B43

Reaction

NAD+
+
[protein]-N6-acetyl-L-lysine
=
nicotinamide
 +
[protein]-L-lysine
 +
2'-O-acetyl-ADP-ribose

Synonyms

CobB, CobB Sir2 protein, histone desuccinylase, hSIRT5, hSIRT6, lysine desuccinylase, mitochondrial NAD+-dependent lysine deacylase, NAD+ dependent deacetylase, NAD+-dependent protein deacetylase, NAD+-dependent protein deacylase, NAD+-dependent sirtuin deacetylase, nicotinamide adenine dinucleotide-dependent protein deacetylase, Sir2Af1, SIRT5, SIRT5iso1, SIRT5iso2, SIRT5iso3, SIRT5iso4, sirtuin 5, sirtuin 5 deacylase, sirtuin 5 lysine deacylase, sirtuin deacylase, sirtuin-5, zSIRT5

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.B43 protein-lysine desuccinylase (NAD+)

Cloned

Cloned on EC 2.3.1.B43 - protein-lysine desuccinylase (NAD+)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
crystals are grown by hanging drop vapor diffusion method, truncated SIRT5(34–302) is cloned and expressed in Escherichia coli
expressed in Escherichia coli BL21(DE3) cells
expression in Escherichia coli
gene SIRT5, alternative splicing of the human SIRT5 mRNA results in two distinct SIRT5 protein isoforms, differing at their C-termini, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3, method, overview)
gene SIRT5, genotyping of the Ctl and OE mice. Sirt5 hepatic overexpressing (ob/ob-SIRT5 OE) mouse containing at least one allele of alternative Sirt5 gene and Alb-cre. The other one is control ob/ob mouse (ob/ob- control) containing only the floxed Sirt5 KI allele
gene SIRT5, quantitative real-time PCR expression analysis
gene SIRT5, recombinant expression of His-tagged enzyme in Escherichia coli strains Rosetta2(DE3) and BL21(DE3)
gene SIRT5, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene SIRT5, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain Transetta(DE3)
gene SIRT5, recombinant expression of N-terminally StrepII-tagged enzyme in Escherichia coli strain BL21(DE3)
gene SIRT5, recombinant expression of tagged SIRT5 in HEK-293T cells, coexpression of wild-type and mutant protein kinase M2s, i.e. wild-type and mutants K62R/E, K135R/E, K311R/E, K498R/E, K311R/K498R, and K311E/K498E. Interactions between endogenous PKM2 and ectopically expressed FLAG-tagged PKM2, including the wild-type PKM2, the K311R mutant, and the succinylation-mimic K311E mutant, overview
gene SIRT5, transient overexpression of wild-type and mutant enzymes in HEK-293T cells or RAW-264.7 cells
overexpression in Escherichia coli BL21
SIRT5 isozymes, DNA and amino acid sequence determination and analysis
the human SIRT5 gene encodes two main SIRT5 isoforms, SIRT5iso1 and SIRT5iso2 comprising 310 amino acids and 299 amino acids respectively, which differ slightly from one other at their C-termini, two other human SIRT5 isoforms (SIRT5iso3 and SIRT5iso4) have been reported. SIRT5iso3 is identical to SIRT5iso1 except that it lacks an internal sequence of 18 amino acids, while in SIRT5iso4 the initial 108 amino acids of SIRT5iso1 are missing, including the mitochondrial localization sequence
wild-type enzyme and active site mutants Y92F and R95M
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