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EC 1.14.13.234 Details
show Brenda entryEC number
1.14.13.234
Accepted name
5a,11a-dehydrotetracycline 5-monooxygenase
Reaction
5a,11a-dehydrotetracycline + NADPH + H+ + O2 = 5a,11a-dehydrooxytetracycline + NADP+ + H2O
Other name(s)
oxyS (gene name),
12-dehydrotetracycline 5-monooxygenase
Systematic name
5a,11a-dehydrotetracycline,NADPH:oxygen oxidoreductase (5-hydroxylating)
Comment
The enzyme, characterized from the bacterium Streptomyces rimosus, is bifunctional, catalysing two successive monooxygenation reactions. It starts by catalysing the stereospecific hydroxylation of anhydrotetracycline at C-6 (EC 1.14.13.38). If the released product is captured by EC 1.3.98.4, 5a,11a-dehydrotetracycline dehydrogenase (OxyR), it is reduced to tetracycline. However, if the released product is recaptured by OxyS, it performs an additional hydroxylation at C-5, producing 5a,11a-dehydrooxytetracycline, which, following the action of OxyR, becomes oxytetracycline.
History
created 2016
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1 Oxidoreductases
1.1 Acting on the CH-OH group of donors
1.4.3.9 created 1972, deleted 1984 1.4.3.17 created 2000, deleted 2003 1.4.3.18 proposed 2000 1.14.13.45 created 1992, deleted 2003
