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EC 1.14.13.109 Details
show Brenda entryEC number
1.14.13.109
Accepted name
abieta-7,13-dien-18-ol hydroxylase
Reaction
abieta-7,13-dien-18-ol + NADPH + H+ + O2 = abieta-7,13-dien-18-al + NADP+ + 2 H2O (overall reaction);;(1a) abieta-7,13-dien-18-ol + NADPH + H+ + O2 = abieta-7,13-dien-18,18-diol + NADP+ + H2O;;(1b) abieta-7,13-dien-18,18-diol = abieta-7,13-dien-18-al + H2O (spontaneous)
Other name(s)
CYP720B1,
PtAO,
abietadienol hydroxylase (ambiguous)
Systematic name
abieta-7,13-dien-18-ol,NADPH:oxygen oxidoreductase (18-hydroxylating)
Comment
A heme-thiolate protein (P-450). This enzyme catalyses a step in the pathway of abietic acid biosynthesis. The activity has been demonstrated in cell-free stem extracts of Abies grandis (grand fir) and Pinus contorta (lodgepole pine) [1], and the gene encoding the enzyme has been identified in Pinus taeda (loblolly pine) [3]. The recombinant enzyme catalyses the oxidation of multiple diterpene alcohol and aldehydes, including levopimaradienol, isopimara-7,15-dienol, isopimara-7,15-dienal, dehydroabietadienol and dehydroabietadienal. It is not able to oxidize abietadiene.
History
created 2009, modified 2012, deleted 2018
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1 Oxidoreductases
1.1 Acting on the CH-OH group of donors
1.4.3.9 created 1972, deleted 1984 1.4.3.17 created 2000, deleted 2003 1.4.3.18 proposed 2000 1.14.13.45 created 1992, deleted 2003
