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2.6.1.B16: amine transaminase

This is an abbreviated version!
For detailed information about amine transaminase, go to the full flat file.

Word Map on EC 2.6.1.B16

Reaction

pyruvate
+
(S)-1-phenylethylamine
=
L-alanine
 +
acetophenone

Synonyms

(S)-amine-transaminase, (S)-ATA, (S)-selective amine transaminase, (S)-selective Chromobacterium violaceum omega-transaminase, (S)-selective omega-transaminase, amine transaminase, amine-transaminases, ATA, ATA_SLM16, Ban-TA, Cv-ATA, Cv-omegaTA, HELO 1904, HEWT, omega-amino acid:pyruvate transaminase, omega-TA, omega-transaminase, Rsp-ATA, SpuC

ECTree

     2 Transferases
         2.6 Transferring nitrogenous groups
             2.6.1 Transaminases
                2.6.1.B16 amine transaminase

Specific Activity

Specific Activity on EC 2.6.1.B16 - amine transaminase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0016
purified recombinant wild-type enzyme, pH 10.0, 30°C, substrates (S)-1-phenylethylamine and 4-phenyl-2-butanone
0.0018
purified recombinant wild-type enzyme, pH 10.0, 30°C, substrates (S)-1-phenylethylamine and rac-2-methylcyclohexanone
0.0019
purified recombinant wild-type enzyme, pH 10.0, 30°C, substrates (S)-1-phenylethylamine and 2-heptanone
0.002
purified recombinant wild-type enzyme, pH 10.0, 30°C, substrates (S)-1-phenylethylamine and cyclohexanone
0.0021
purified recombinant wild-type enzyme, pH 10.0, 30°C, substrates (S)-1-phenylethylamine and 2-butanone
0.0028
purified recombinant wild-type enzyme, pH 10.0, 30°C, substrates (S)-1-phenylethylamine and methoxyacetone
0.0038
purified recombinant wild-type enzyme, pH 10.0, 30°C, substrates (S)-1-phenylethylamine and cyclooctanone
0.0065
-
pH 8.0, 30°C, substrate rac-2,2-dimethyl-1-phenylpropan-1-amine, mutant Y59W/Y87V/T231A
0.0067
purified recombinant wild-type enzyme, pH 10.0, 30°C, substrates (S)-1-phenylethylamine and beta-tetralone
0.007
purified recombinant wild-type enzyme, pH 10.0, 30°C, substrates (S)-1-phenylethylamine and oxaloacetate
0.0115
-
pH 8.0, 30°C, substrate rac-2,2-dimethyl-1-phenylpropan-1-amine, mutant Y59W/Y87L/T231A
0.019
-
pH 8.0, 30°C, substrate rac-2,2-dimethyl-1-phenylpropan-1-amine, mutant Y59W/Y87L/T231A/L382M
0.0324
-
pH 8.0, 30°C, substrate rac-2,2-dimethyl-1-phenylpropan-1-amine, mutant Y59W/Y87L/T231A/P281S/G429A
0.0448
-
pH 8.0, 30°C, substrate rac-2,2-dimethyl-1-phenylpropan-1-amine, mutant Y59W/Y87L/T231A/G429A
0.06
-
recombinant mutant F84A, pH 8.0, 25°C
0.0771
-
pH 8.0, 30°C, substrate rac-2,2-dimethyl-1-phenylpropan-1-amine, mutant Y59W/Y87L/T231A/L382M/G429A
0.43
-
recombinant mutant I258A, pH 8.0, 25°C
0.5
-
recombinant mutant Y149F, pH 8.0, 25°C
0.58
substrate benzaldehyde, cosubstrate isopropylamine, pH 8.0, 37°C
0.94
purified recombinant wild-type enzyme, pH 10.0, 30°C, substrates (S)-1-phenylethylamine and pyruvate or methylpyruvate
1.3
purified recombinant wild-type enzyme, pH 10.0, 30°C, substrates (S)-1-phenylethylamine and 2-oxoglutarate
2.5
purified recombinant wild-type enzyme, pH 10.0, 30°C, substrates beta-alanine and pyruvate
3.99
-
recombinant wild-type enzyme, pH 8.0, 25°C
33
-
purified recombinant enzyme, pH 9.5, 65°C, substrate (S)-alpha-methylbenzylamine
4.43
-
recombinant mutant W56G, pH 8.0, 25°C
6
-
purified recombinant enzyme, pH 9.0, 50°C, substrate (S)-alpha-methylbenzylamine