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2.6.1.B16: amine transaminase

This is an abbreviated version!
For detailed information about amine transaminase, go to the full flat file.

Word Map on EC 2.6.1.B16

Reaction

pyruvate
+
(S)-1-phenylethylamine
=
L-alanine
+
acetophenone

Synonyms

(S)-amine-transaminase, (S)-ATA, (S)-selective amine transaminase, (S)-selective Chromobacterium violaceum omega-transaminase, (S)-selective omega-transaminase, amine transaminase, amine-transaminases, ATA, ATA_SLM16, Ban-TA, Cv-ATA, Cv-omegaTA, HELO 1904, HEWT, omega-amino acid:pyruvate transaminase, omega-TA, omega-transaminase, Rsp-ATA, SpuC

ECTree

     2 Transferases
         2.6 Transferring nitrogenous groups
             2.6.1 Transaminases
                2.6.1.B16 amine transaminase

General Stability

General Stability on EC 2.6.1.B16 - amine transaminase

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
in 1 M KCl, 50% residual activity after 24 h
in 1 M NaCl, 40% residual activity after 24 h
the enzyme at concentrations greater than 5mM of cofactor pyridoxal 5'-phosphate loses the activity significantly faster than 0.1mM, confirming the destabilization effect
the HEWT quaternary structure is quite unstable
-
the monomer-dimer equilibrium has an effect on enzyme storage stability. After 3-4 days of incubation, the enzyme activity starts to decrease at the same time as monomers start to show up in the BN-PAGE
-