Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

2.6.1.B16: amine transaminase

This is an abbreviated version!
For detailed information about amine transaminase, go to the full flat file.

Word Map on EC 2.6.1.B16

Reaction

pyruvate
+
(S)-1-phenylethylamine
=
L-alanine
+
acetophenone

Synonyms

(S)-amine-transaminase, (S)-ATA, (S)-selective amine transaminase, (S)-selective Chromobacterium violaceum omega-transaminase, (S)-selective omega-transaminase, amine transaminase, amine-transaminases, ATA, ATA_SLM16, Ban-TA, Cv-ATA, Cv-omegaTA, HELO 1904, HEWT, omega-amino acid:pyruvate transaminase, omega-TA, omega-transaminase, Rsp-ATA, SpuC

ECTree

     2 Transferases
         2.6 Transferring nitrogenous groups
             2.6.1 Transaminases
                2.6.1.B16 amine transaminase

Purification

Purification on EC 2.6.1.B16 - amine transaminase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme from Escherichia coli strain BL21(C2530) by centrifugation at 30000 x g for 10 min, heat treatment at 70°C for 10 min, ultrafiltration, and gel filtration
-
recombinant N-terminally His6-tagged enzyme from Escherichia coli BL21(DE3) by nickel affinity chromatography and desalting gel filtration
-