2.3.1.253: phloroglucinol synthase
This is an abbreviated version!
For detailed information about phloroglucinol synthase, go to the full flat file.
Reaction
3 malonyl-CoA = + 3 CO2 + 3 CoA
Synonyms
PhlD, phloroglucinol synthase, type III polyketide synthase
ECTree
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Engineering
Engineering on EC 2.3.1.253 - phloroglucinol synthase
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A289R
the mutant shows 1.4fold improved catalytic efficiency compared to the wild type enzyme
A60L
the mutant still retains the wild type activity to react with lauroyl-CoA
H24L
the mutant shows reduced reactivity toward lauroyl-CoA and has lost the ability to produce phloroglucinol
H24V
the mutation significantly reduces the reactivity of the enzyme with lauroyl-CoA while still retaining its physiological activity to synthesize phloroglucinol
K210L
the mutant shows 1.9fold improved catalytic efficiency compared to the wild type enzyme
K210L/Y256R
the mutant shows 0.9fold improved catalytic efficiency compared to the wild type enzyme
L59M
the mutation significantly reduces the reactivity of the enzyme with lauroyl-CoA while still retaining its physiological activity to synthesize phloroglucinol
M21I
the mutation significantly reduces the reactivity of the enzyme with lauroyl-CoA while still retaining its physiological activity to synthesize phloroglucinol
M21T
M21T/L54V/A82T/S96R/A181S
-
the mutant shows 110% of wild type specific activity
M21T/N27D/A82T/A181S
-
the mutant shows 90% of wild type specific activity
M21T/N27D/L54V/A82T/A181S/S243T
-
the mutant shows wild type specific activity
M21T/N27D/L54V/A82T/L136M/A181S/S243T
-
the mutant shows 110% of wild type specific activity
Y256R
the mutant shows 1.9fold improved catalytic efficiency compared to the wild type enzyme
Y256R/A289R
the mutant shows 0.8fold improved catalytic efficiency compared to the wild type enzyme
A289R
-
the mutant shows 1.4fold improved catalytic efficiency compared to the wild type enzyme
-
H24L
-
the mutant shows reduced reactivity toward lauroyl-CoA and has lost the ability to produce phloroglucinol
-
H24V
-
the mutation significantly reduces the reactivity of the enzyme with lauroyl-CoA while still retaining its physiological activity to synthesize phloroglucinol
-
K210L
-
the mutant shows 1.9fold improved catalytic efficiency compared to the wild type enzyme
-
K210L/Y256R
-
the mutant shows 0.9fold improved catalytic efficiency compared to the wild type enzyme
-
L59M
-
the mutation significantly reduces the reactivity of the enzyme with lauroyl-CoA while still retaining its physiological activity to synthesize phloroglucinol
-
M21I
-
the mutation significantly reduces the reactivity of the enzyme with lauroyl-CoA while still retaining its physiological activity to synthesize phloroglucinol
-
M21T
-
the mutant still retains the wild type activity to react with lauroyl-CoA
-
Y256R
-
the mutant shows 1.9fold improved catalytic efficiency compared to the wild type enzyme
-
Y256R/A289R
-
the mutant shows 0.8fold improved catalytic efficiency compared to the wild type enzyme
-
M21T
the mutant still retains the wild type activity to react with lauroyl-CoA