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2.3.1.255: N-terminal amino-acid Nalpha-acetyltransferase NatA

This is an abbreviated version!
For detailed information about N-terminal amino-acid Nalpha-acetyltransferase NatA, go to the full flat file.

Word Map on EC 2.3.1.255

Reaction

acetyl-CoA
+
an N-terminal-glycyl-[protein]
=
an N-terminal-Nalpha-acetyl-glycyl-[protein]
 +
CoA

Synonyms

ARD homolog a, ARD1, ARD1-NAT1 Nalpha-acetyltransferase, ARD1131, ARD1198, ARD1225, ARD1235, Ard1b, ARD1p, ARD2, arrest defective 1, arrest-defective protein 1, arrest-defective protein 1 homologue, AtNAA10, daf-31, DXS707, EC 2.3.1.88, hARD1, hNaa10, hNaa15, hNaa16p, hNat2, More, MtRimI, MUSE6, N(alpha)-acetyltransferase 10, N-alpha-acetyltransferase, N-alpha-acetyltransferase 10, N-alpha-acetyltransferase 11, N-alpha-acetyltransferase 15, N-terminal acetyltransferase, N-terminal acetyltransferase 10, N-terminal acetyltransferase A, N-terminal protein acetyltransferase, NAA10, NAA10-NAA15, NAA10-NAA15 complex, NAA10-NAA15 protein complex, NAA10/ARD1, NAA11, NAA15, Nalpha-acetyltransferase, Nalpha-acetyltransferase 10, NAT, NAT1, NatA, NatA acetyltransferase, NatA Nalpha-terminal acetyltransferase, NatA protein complex, NatA protein Nalpha-terminal-acetyltransferase complex, RimI, RimI acetyltransferase, Rv3420c, SCNaa15, ScNatA, SpNaa10, SpNaa15, SSO0209, Ta0058, Ta1140, TbARD1, TcNatA, TE2, vnc, yNaa10, yNaa15, zNaa10

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.255 N-terminal amino-acid Nalpha-acetyltransferase NatA

Purification

Purification on EC 2.3.1.255 - N-terminal amino-acid Nalpha-acetyltransferase NatA

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
HisTrap column chromatography and Superdex 75 gel filtration
Ni-NTA resin column chromatography and Superdex 200 gel filtration
Ni-NTA resin column chromatography, Q ion exchange column chromatography, and S200 gel filtration
-
Ni-Sepharose column chromatography and Sephacryl S-200 gel filtration
recombinant C-terminally His6-tagged enzyme RimI from Escherichia coli by nickel affinity and gel filtration
recombinant GST-tagged wild-type and mutant Naa10 enzymes from Escherichia coli strain BL21 by glutathione affinity chromatography
recombinant His-MBP-tagged wild-type and mutant Naa10 from Escherichia coli strain BL21 Star DE3 by affinity chromatography
P41227; Q9BXJ9
recombinant His-tagged and maltose binding protein-tagged subunits hNaa15p, hNaa10p, and hNaa50p from Escherichia coli by nickel affinity chromatography
-
recombinant His-tagged ARD1/Naa10 from Escherichia coli by nickel affinity chromatography, anion exchange chromatography, and gel filtration. After purification, rhARD1/NAA10 mainly exists in a high oligomeric state and has only a few monomers. Recombinant GST-tagged enzyme from Escherichia coli strain BL21 by glutathione affinity chromatography
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
recombinant His-tagged hARD1/NAA10 enzyme by nickel affinity chromatography, with or without anion exchange chromatography, followed by gel filtration, and dialysis, rhARD1/NAA10 aggregates during purification
recombinant His-tagged hNatA by affinity chromatography and gel filtration
P41227; Q9BXJ9
Resource Q column chromatography, Mono Q column chromatography, and Superdex 200 gel filtration
-
ribosomes of HEK-293 cells are isolated
-