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2.3.1.255: N-terminal amino-acid Nalpha-acetyltransferase NatA

This is an abbreviated version!
For detailed information about N-terminal amino-acid Nalpha-acetyltransferase NatA, go to the full flat file.

Word Map on EC 2.3.1.255

Reaction

acetyl-CoA
+
an N-terminal-glycyl-[protein]
=
an N-terminal-Nalpha-acetyl-glycyl-[protein]
 +
CoA

Synonyms

ARD homolog a, ARD1, ARD1-NAT1 Nalpha-acetyltransferase, ARD1131, ARD1198, ARD1225, ARD1235, Ard1b, ARD1p, ARD2, arrest defective 1, arrest-defective protein 1, arrest-defective protein 1 homologue, AtNAA10, daf-31, DXS707, EC 2.3.1.88, hARD1, hNaa10, hNaa15, hNaa16p, hNat2, More, MtRimI, MUSE6, N(alpha)-acetyltransferase 10, N-alpha-acetyltransferase, N-alpha-acetyltransferase 10, N-alpha-acetyltransferase 11, N-alpha-acetyltransferase 15, N-terminal acetyltransferase, N-terminal acetyltransferase 10, N-terminal acetyltransferase A, N-terminal protein acetyltransferase, NAA10, NAA10-NAA15, NAA10-NAA15 complex, NAA10-NAA15 protein complex, NAA10/ARD1, NAA11, NAA15, Nalpha-acetyltransferase, Nalpha-acetyltransferase 10, NAT, NAT1, NatA, NatA acetyltransferase, NatA Nalpha-terminal acetyltransferase, NatA protein complex, NatA protein Nalpha-terminal-acetyltransferase complex, RimI, RimI acetyltransferase, Rv3420c, SCNaa15, ScNatA, SpNaa10, SpNaa15, SSO0209, Ta0058, Ta1140, TbARD1, TcNatA, TE2, vnc, yNaa10, yNaa15, zNaa10

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.255 N-terminal amino-acid Nalpha-acetyltransferase NatA

Cloned

Cloned on EC 2.3.1.255 - N-terminal amino-acid Nalpha-acetyltransferase NatA

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a plasmid expressing hNaa16p, NARG1L-FLAG, is used
-
cloning of V5-tagged hNaa15p, hNaa25p, and hNaa35p from HEK-293 cell genomic DNA, expression of His-tagged and maltose binding protein-tagged subunits hNaa15p, hNaa10p, and hNaa50p in Escherichia coli, efficient expression of MBP-hNaa15p requires coexpression of pDC952, a plasmid carrying the Escherichia coli argU gene
-
expressed in Escherichia coli BL21 cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3)pLysS cells
expressed in Escherichia coli Rosetta (DE3)pLysS cells
-
expressed in Escherichia coli Rosetta II(DE3)pLysS cells
expressed in Escherichia coli Rosetta Star cells
-
gene NAA10, DNA and amino acid sequence determination and analysis, genotyping, recombinant expression of His-MBP-tagged wild-type and mutant Naa10 in Escherichia coli strain BL21 Star DE3
P41227; Q9BXJ9
gene NAA10, genotyping, recombinant expression of His-MBP-tagged wild-type and mutant Naa10 in Escherichia coli and in HeLa cells
P41227; Q9BXJ9
gene NAA10, genotyping, sequence comparisons, enzyme expression analysis
gene NAA10, real-time reverse transcription-PCR enzyme expression analysis
gene NAA10, recombinant expression of His-tagged ARD1/Naa10 in Escherichia coli strain BL21, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21
gene NAA10, recombinant expression of the enzyme in human 293T cells using a lentiviral vector, recombinant expression of GST-tagged wild-type and mutant Naa10 enzymes in Escherichia coli strain BL21
gene rimI, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene Rv3420c or rimI, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli
genes NAA10 and NAA15, recombinant enzyme complex NatA expression in Spodoptera frugiperda SF9 cells via transfection using the baculovirus system
P07347; P12945
genes TcNaa15 and TcNaa10, DNA and amino acid sequence determination, comparison, and analysis
Q4DSU6; Q4D0P4
recombinant expression of His-tagged hARD1/NAA10
recombinant expression of N-terminally His-tagged NatA in Spodoptera frugiperda Sf9 cells, coexpression of tagged HYPK
P41227; Q9BXJ9