EC Explorer

1.14.19.55

4-hydroxybenzoate brominase (decarboxylating)

(1) 4-hydroxybenzoate + 2 NADPH + 2 bromide + 2 O₂ + 2 H⁺ = 2,4-dibromophenol + 2 NADP⁺ + CO₂ + 4 H₂O (overall reaction);;(1a) 4-hydroxybenzoate + NADPH + bromide + O₂ + H⁺ = 3-bromo-4-hydroxybenzoate + NADP⁺ + 2 H₂O;;(1b) 3-bromo-4-hydroxybenzoate + NADPH + bromide + O₂ + H⁺ = 2,4-dibromophenol + NADP⁺ + CO₂ + 2 H₂O;;(2) 3,4-dihydroxybenzoate + 2 NADPH + 2 bromide + 2 O₂ + 2 H⁺ = 3,5-dibromobenzene-1,2-diol + 2 NADP⁺ + CO₂ + 4 H₂O (overall reaction);;(2a) 3,4-dihydroxybenzoate + NADPH + bromide + O₂ + H⁺ = 3-bromo-4,5-dihydroxybenzoate + NADP⁺ + 2 H₂O;;(2b) 3-bromo-4,5-dihydroxybenzoate + NADPH + bromide + O₂ + H⁺ = 3,5-dibromobenzene-1,2-diol + NADP⁺ + CO₂ + 2 H₂O

bmp5 (gene name)

4-hydroxybenzoate:NADPH oxidoreductase (brominating, decarboxylating)

Contains FAD. The enzyme, described from epiphytic marine bacteria of the genera Pseudoalteromonas and Marinomonas, is an unusual single-component FAD-dependent halogenase that contains a distinct NAD(P)H binding domain and does not require an additional flavin reductase for activity. The enzyme catalyses a bromination of its substrate, followed by a second bromination concurrent with decarboxylation.

created 2018