2.4.1.362: alpha-(1->3) branching sucrase
This is an abbreviated version!
For detailed information about alpha-(1->3) branching sucrase, go to the full flat file.
Word Map on EC 2.4.1.362
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2.4.1.362
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platelet
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dextrans
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ringer
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bicarbonate
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transfusions
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glucosylation
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allergic
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apheresis
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transfused
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hypotonic
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processor
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leuconostoc
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acid-citrate-dextrose
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comb-like
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resuspended
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gorge
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gluco-oligosaccharides
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citreum
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volume-reduced
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glucansucrases
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synthesis
- 2.4.1.362
- platelet
- dextrans
-
ringer
- bicarbonate
-
transfusions
-
glucosylation
-
allergic
-
apheresis
-
transfused
-
hypotonic
-
processor
- leuconostoc
-
acid-citrate-dextrose
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comb-like
-
resuspended
-
gorge
- gluco-oligosaccharides
- citreum
-
volume-reduced
- glucansucrases
- synthesis
Reaction
Synonyms
branching sucrase, branching sucrase A, branching sucrase B, BRS-A, BRS-B, BRS-BDELTA1, brsA, BrsB, GtfZ
ECTree
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Substrates Products
Substrates Products on EC 2.4.1.362 - alpha-(1->3) branching sucrase
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REACTION DIAGRAM
isomalto/malto-polysaccharide + sucrose
D-fructose + isomalto/malto-polysaccharide containing a (1->3)-alpha-D-glucose branch
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alpha(1->3)-branched IMMP, IMMP Mw is 70 kDa, transglucosylation activity
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?
sucrose + (1->6)-alpha-D-glucan
D-fructose + (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch
sucrose + dextran
D-fructose + a (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch
D-fructose + dextran containing a (1->3)-alpha-D-glucose branch
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dextran Mw is 70 kDa, transglucosylation activity
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?
dextran + sucrose
D-fructose + dextran containing a (1->3)-alpha-D-glucose branch
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dextran Mw is 70 kDa, transglucosylation activity
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?
D-fructose + (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch
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?
sucrose + (1->6)-alpha-D-glucan
D-fructose + (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch
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transglucosylation activity
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?
sucrose + (1->6)-alpha-D-glucan
D-fructose + (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch
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?
sucrose + (1->6)-alpha-D-glucan
D-fructose + (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch
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transglucosylation activity
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?
sucrose + (1->6)-alpha-D-glucan
D-fructose + (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch
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?
sucrose + (1->6)-alpha-D-glucan
D-fructose + (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch
Leuconostoc citreum NRRL B-1299
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?
sucrose + (1->6)-alpha-D-glucan
D-fructose + (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch
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?
D-fructose + a (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch
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dextran of 70 kDa
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-
?
sucrose + dextran
D-fructose + a (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch
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dextran of 70 kDa
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-
?
sucrose + dextran
D-fructose + a (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch
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-
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?
sucrose + dextran
D-fructose + a (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch
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dextran of 70 kDa
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-
?
sucrose + dextran
D-fructose + a (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch
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-
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?
sucrose + dextran
D-fructose + a (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch
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dextran of 70 kDa
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-
?
sucrose + H2O
D-fructose + D-glucose
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sucrose hydrolysis
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?
?
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domain CD2 additionally displays hydrolytic acivity on dextran
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?
additional information
?
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enzyme harbors two separate catalytic cores (CD1 and CD2), predicted to have glucansucrase and branching sucrase specificity, respectively. CD2 displays an efficient transglycosidase activity with sucrose as donor substrate. The CD2-catalyzed transglycosylation reaction follows a Ping Pong Bi Bi mechanism and produces single glucosyl (alpha1->3) linked branches onto dextran, resulting in the production of highly branched comb-like alpha-glucan products
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?
additional information
?
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enzyme GtfZ-D2 produces partially branched polymers. Analysis of the glucosyl linkage composition of the (alpha1->3)-branched polymers. Reaction product analyses with 1D 1H NMR, overview. GtfZ-CD2 of Lactobacillus kunkeei uses sucrose as a glucose donor to decorate dextran molecules, adding single (alpha1->3)-branched glucose units on the linear (alpha1->6) glucose chain. GtfZ-CD2 also exhibits (alpha1->3)-branching activity on IMMP (a product of reaction of GtfB, EC 2.4.1.B52, with about 90% (alpha1->6)-linkages)
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additional information
?
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domain CD2 additionally displays hydrolytic acivity on dextran
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?
additional information
?
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enzyme harbors two separate catalytic cores (CD1 and CD2), predicted to have glucansucrase and branching sucrase specificity, respectively. CD2 displays an efficient transglycosidase activity with sucrose as donor substrate. The CD2-catalyzed transglycosylation reaction follows a Ping Pong Bi Bi mechanism and produces single glucosyl (alpha1->3) linked branches onto dextran, resulting in the production of highly branched comb-like alpha-glucan products
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?
additional information
?
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enzyme GtfZ-D2 produces partially branched polymers. Analysis of the glucosyl linkage composition of the (alpha1->3)-branched polymers. Reaction product analyses with 1D 1H NMR, overview. GtfZ-CD2 of Lactobacillus kunkeei uses sucrose as a glucose donor to decorate dextran molecules, adding single (alpha1->3)-branched glucose units on the linear (alpha1->6) glucose chain. GtfZ-CD2 also exhibits (alpha1->3)-branching activity on IMMP (a product of reaction of GtfB, EC 2.4.1.B52, with about 90% (alpha1->6)-linkages)
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additional information
?
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alpha(1->2) or alpha(1->3) branched dextrans with high molar masses and controlled architecture are synthesized using dextransucrase from Oenococcous kitahare DSM 17330 (DSR-OK), the branching sucrase from Leuconostoc citreum NRRL B-1299 (BRS-A) and the branching sucrase from Leuconostoc citreum NRRL B-742 (BRS-BDELTA1), all in cell-free extract from recombinant Escherichia coli strain BL21. Their molecular structure, solubility, conformation, film-forming ability, as well as their thermal and mechanical properties are determined, detailed overview. The pattern of the molar mass increase depends on the branching degree and the branching type, probably because of the different conformations due to the different flexibility and hindrance introduced by either the alpha(1->2) or the alpha(1->3) linkages
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additional information
?
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Leuconostoc citreum NRRL B-1299
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alpha(1->2) or alpha(1->3) branched dextrans with high molar masses and controlled architecture are synthesized using dextransucrase from Oenococcous kitahare DSM 17330 (DSR-OK), the branching sucrase from Leuconostoc citreum NRRL B-1299 (BRS-A) and the branching sucrase from Leuconostoc citreum NRRL B-742 (BRS-BDELTA1), all in cell-free extract from recombinant Escherichia coli strain BL21. Their molecular structure, solubility, conformation, film-forming ability, as well as their thermal and mechanical properties are determined, detailed overview. The pattern of the molar mass increase depends on the branching degree and the branching type, probably because of the different conformations due to the different flexibility and hindrance introduced by either the alpha(1->2) or the alpha(1->3) linkages
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-
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additional information
?
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alpha(1->2) or alpha(1->3) branched dextrans with high molar masses and controlled architecture are synthesized using dextransucrase from Oenococcous kitahare DSM 17330 (DSR-OK), the branching sucrase from Leuconostoc citreum NRRL B-1299 (BRS-A) and the branching sucrase from Leuconostoc citreum NRRL B-742 (BRS-BDELTA1), all in cell-free extract from recombinant Escherichia coli strain BL21. Their molecular structure, solubility, conformation, film-forming ability, as well as their thermal and mechanical properties are determined, detailed overview. The pattern of the molar mass increase depends on the branching degree and the branching type, probably because of the different conformations due to the different flexibility and hindrance introduced by either the alpha(1->2) or the alpha(1->3) linkages
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