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Literature summary for 2.4.1.362 extracted from

  • Meng, X.; Gangoiti, J.; Wang, X.; Grijpstra, P.; van Leeuwen, S.; Pijning, T.; Dijkhuizen, L.
    Biochemical characterization of a GH70 protein from Lactobacillus kunkeei DSM 12361 with two catalytic domains involving branching sucrase activity (2018), Appl. Microbiol. Biotechnol., 102, 7935-7950 .
No PubMed abstract available
Search for more literature for 2.4.1.362

Activating Compound

Activating Compound Comment Organism Structure
Dextran presence of dextran highly stimulates the activity of domain CD2 Apilactobacillus kunkeei

Cloned(Commentary)

Cloned (Comment) Organism
expression of domain CD2, amino acid residues 1212-2264 of the enzyme Apilactobacillus kunkeei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
126.7
-
 sucrose pH 5.5, 30°C Apilactobacillus kunkeei
251.6
-
 Dextran pH 5.5, 30°C Apilactobacillus kunkeei

Organism

Organism UniProt Comment Textmining
Apilactobacillus kunkeei
-
-
-
Apilactobacillus kunkeei DSM 12361
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
34.3
-
 transferase activity, pH 5.5, 30°C Apilactobacillus kunkeei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information domain CD2 additionally displays hydrolytic acivity on dextran Apilactobacillus kunkeei ?
-
 ?
additional information enzyme harbors two separate catalytic cores (CD1 and CD2), predicted to have glucansucrase and branching sucrase specificity, respectively. CD2 displays an efficient transglycosidase activity with sucrose as donor substrate. The CD2-catalyzed transglycosylation reaction follows a Ping Pong Bi Bi mechanism and produces single glucosyl (alpha1->3) linked branches onto dextran, resulting in the production of highly branched comb-like alpha-glucan products Apilactobacillus kunkeei ?
-
 ?
additional information domain CD2 additionally displays hydrolytic acivity on dextran Apilactobacillus kunkeei DSM 12361 ?
-
 ?
additional information enzyme harbors two separate catalytic cores (CD1 and CD2), predicted to have glucansucrase and branching sucrase specificity, respectively. CD2 displays an efficient transglycosidase activity with sucrose as donor substrate. The CD2-catalyzed transglycosylation reaction follows a Ping Pong Bi Bi mechanism and produces single glucosyl (alpha1->3) linked branches onto dextran, resulting in the production of highly branched comb-like alpha-glucan products Apilactobacillus kunkeei DSM 12361 ?
-
 ?
sucrose + dextran dextran of 70 kDa Apilactobacillus kunkeei D-fructose + a (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch
-
 ?
sucrose + dextran dextran of 70 kDa Apilactobacillus kunkeei DSM 12361 D-fructose + a (1->6)-alpha-D-glucan containing a (1->3)-alpha-D-glucose branch
-
 ?

Subunits

Subunits Comment Organism
? x * 130000, isolated domain CD2, SDS-PAGE, x * 121000, isolated domain CD2, calculated from sequence Apilactobacillus kunkeei

Synonyms

Synonyms Comment Organism
GtfZ
-
 Apilactobacillus kunkeei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 isolated domain CD2 Apilactobacillus kunkeei

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 8 h, 90% residual activity, isolated domain CD2 Apilactobacillus kunkeei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 isolated domain CD2 Apilactobacillus kunkeei