2.4.1.362: alpha-(1->3) branching sucrase

This is an abbreviated version!
For detailed information about alpha-(1->3) branching sucrase, go to the full flat file.

branching sucrase, branching sucrase A, branching sucrase B, BRS-A, BRS-B, BRS-BDELTA1, brsA, BrsB, GtfZ

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Results	in table
1	Activating Compound
5	Application
4	Cloned(Commentary)
8	General Information
4	KM Value [mM]
5	Natural Substrates/ Products (Substrates)
21	Organism
4	pH Optimum
4	Protein Variants
2	Purification (Commentary)
1	Reaction
10	Reference
1	Specific Activity [micromol/min/mg]
29	Substrates and Products (Substrate)
2	Subunits
19	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
2	Temperature Stability [°C]
2	Turnover Number [1/s]

Cloned on EC 2.4.1.362 - alpha-(1->3) branching sucrase

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expression in Escherichia coli

expression of domain CD2, amino acid residues 1212-2264 of the enzyme

gene gtfB, recombinant expression of C-terminally His-tagged enzyme mutant GtfZ-CD2 in Escherichia coli strain BL21(DE3)

recombinant expression of BRS-BDELTA1 enzyme in Escherichia coli strain BL21