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2.3.1.85: fatty-acid synthase system

This is an abbreviated version!
For detailed information about fatty-acid synthase system, go to the full flat file.

Word Map on EC 2.3.1.85

Reaction

acetyl-CoA
+ 7 malonyl-CoA + 14 NADPH + 14 H+ =
hexadecanoate
 + 8 CoA + 7 CO2 + 14 NADP+ + 6 H2O

Synonyms

F09E10.3 protein, FAS, FAS-II, FASI, FASII, FASN, fatty acid synthase, fatty acid synthase I, fatty acid synthase II, fatty acid synthase type 2, fatty-acid synthase, type 2 dissociative FAS, type 2 fatty acid synthase, type II fatty acid synthase, yeast fatty acid synthase

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.85 fatty-acid synthase system

Engineering

Engineering on EC 2.3.1.85 - fatty-acid synthase system

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D2553A/G2559S/M2600W
-
in addition to G2559S/M2600W, mutation in ketoacyl synthase domain KS, mutation increases the fraction of long chain acyl-CoA produced
D2553N/G2559S/M2600W
-
in addition to G2559S/M2600W, mutation in ketoacyl synthase domain KS, mutation increases the fraction of long chain acyl-CoA produced
D2556A/G2559S/M2600W
-
in addition to G2559S/M2600W, mutation in ketoacyl synthase domain KS, mutation increases the fraction of long chain acyl-CoA produced
G2559S/D2622A/M2600W
-
in addition to G2559S/M2600W, mutation in ketoacyl synthase domain KS, mutant is most efficient in C8-CoA production but impaired in activity
G2559S/M2600W
-
mutant produces produces a bimodal spectrum of C8- and C14/C16-CoA in vitro
G2559S/N2621D/M2600W
-
in addition to G2559S/M2600W, mutation in ketoacyl synthase domain KS, mutant is most efficient in C8-CoA production but impaired in activity
N2557E/G2559S/M2600W
-
in addition to G2559S/M2600W, mutation in ketoacyl synthase domain KS, mutant is most efficient in C8-CoA production but impaired in activity
S36T
-
mutant in acyl carrier protein, mutant is incapable of undergoing phosphopantetheinylation. The S36T mutant is a weaker inhibitor of the fatty acid synthase than holo-acyl carrier protein, suggesting that the prostheticgroup of the acyl carrier protein contributes directly to its inhibitory characteristics at high concentrations
A2419L
97% reduction of thioesterase activity
A2419M
92% reduction of thioesterase activity
F2371W
20% reduction of thioesterase activity
F2423A
67% reduction of thioesterase activity
F2423W
24% reduction of thioesterase activity
I2250W
97% reduction of thioesterase activity
K1699A
-
specific activity is 10% of wild-type value
K1699Q
-
specific activity is 7.5% of wild-type value
K2426A
99% reduction of thioesterase activity
S2151A
-
inactive enzyme
S2422A
7% reduction of thioesterase activity
R606A
active site mutant of didomain construct. The specificity constant for malonyl-CoA is 40fold smaller than the wild-type constant
C161A
-
no beta-ketoacyl synthase activity
C161Q
-
mutation of ketoacyl synthase, less conformational variability than wild-type
C161T
-
defective in beta-ketoacyl synthase, no overall fatty acid synthase activity
G1886F
-
beta-ketoreductase activity, 130fold increase in Km-value
G1888A
K326A
R1508A
-
similar activity as wild-type in overall reaction, analysis of partial reactions
R1508D
-
14% of wild-type activity in overall reaction, analysis of partial reactions
R1508K
-
58% of wild-type activity in overall reaction, analysis of partial reactions
R606A
-
reduced malonyl/acetyltransferase activity, increased transacylase activity, 16000fold increased selectivity for acetyl-CoA, 8.5fold increase of Km for malonyl-CoA
R606K
-
reduced malonyl/acetyltransferase activity, increased transacylase activity, 16fold increased selectivity for acetyl-CoA
S215A
-
defective in acyl carrier protein
S2302A
-
mutation of thioesterase, less conformational variability than wild-type
S518A
-
no malonyl/acetyltransferase activity
additional information