1.14.99.69: tRNA 2-(methylsulfanyl)-N6-isopentenyladenosine37 hydroxylase
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For detailed information about tRNA 2-(methylsulfanyl)-N6-isopentenyladenosine37 hydroxylase, go to the full flat file.
Reaction
Synonyms
miaE, PP2188, tRNA 2-(methylsulfanyl)-N6-dimethylallyladenosine37 hydroxylase, tRNA 2-methylthio-N6-isopentenyl adenosine(37) hydroxylase
ECTree
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General Information
General Information on EC 1.14.99.69 - tRNA 2-(methylsulfanyl)-N6-isopentenyladenosine37 hydroxylase
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physiological function
binding of tRNA to MiaE induces a protein conformational change that influences the electronic structure of the diiron site analogous to what has been observed for various bacterial multicomponent diiron monooxygenases
physiological function
gene disruption mutants show normal growth characteristics after being shifted from anaerobic to aerobic conditions. Mutants cannot utilize the citric acid cycle intermediates malate, fumarate, and succinate as carbon sources
physiological function
in mutants of MiaE ms2i6A hydroxylation is blocked. Mutant strains are unable to grow aerobically on the dicarboxylic acids of the citric acid cycle, but have normal uptake of dicarboxylic acids and functional enzymes of the citric acid cycle and the aerobic respiratory chain. The ability of S. typhimurium to grow on succinate, fumarate, and malate is dependent on the state of modification in position 37 of those tRNAs normally having ms2io6A37 and is not due to a second cellular function of tRNA (ms2io6A37)hydroxylase, the miaE gene product