1.14.19.58: tryptophan 5-halogenase
This is an abbreviated version!
For detailed information about tryptophan 5-halogenase, go to the full flat file.
Word Map on EC 1.14.19.58
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1.14.19.58
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halogenases
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flavin-dependent
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regioselective
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7-halogenase
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chlorinate
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indole
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synthesis
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albogriseolus
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biocatalysts
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brominate
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cl
-
flavin
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pharmacology
- 1.14.19.58
- halogenases
-
flavin-dependent
-
regioselective
-
7-halogenase
-
chlorinate
- indole
- synthesis
- albogriseolus
-
biocatalysts
-
brominate
- cl
- flavin
- pharmacology
Reaction
Synonyms
halogenase, mibH, pyrH, sttH, trp-5-halogenase
ECTree
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Crystallization
Crystallization on EC 1.14.19.58 - tryptophan 5-halogenase
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purified recombinant His6-tagged enzyme with bound FAD, hanging drop vapour diffusion method, mixing of 22 mg/ml protein in 20 mM HEPES, pH 8.0, and 150 mM KCl, with reservoir solution containing 12.5% MPD, 12.5% PEG 3350, 0.1 M MES/imidazole, pH 6.5, and 0.09 M NPS (1:1:1 NaNO3, Na2HPO4, (NH4)2SO4), X-ray diffraction structure determination and analysis at 1.85 A resolution
sitting-drop vapor-diffusion method at 20°C, crystal structure of a tryptophan 5-halogenase (PyrH) bound to tryptophan and FAD