2.6.1.B18: archeosine synthase (ammonia)

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For detailed information about archeosine synthase (ammonia), go to the full flat file.

Reaction

Synonyms

amidinotransferase QueF-Like, ammonium:preQ0-tRNA aminotransferase, Pcal_0221, QueF-L, QueF-like

ECTree

     2 Transferases

         2.6 Transferring nitrogenous groups

             2.6.1 Transaminases

                2.6.1.B18 archeosine synthase (ammonia)

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Results	in table
1	AA Sequence
3	Cloned(Commentary)
2	Crystallization (Commentary)
24	General Information
6	Natural Substrates/ Products (Substrates)
18	Organism
2	Purification (Commentary)
4	Reaction
8	Reference
16	Substrates and Products (Substrate)
3	Subunits
24	Synonyms
1	Systematic Name

Purification

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Purification on EC 2.6.1.B18 - archeosine synthase (ammonia)

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

recombinant His6-tagged enzyme from Escherichia coli by heat treatment at 80°C and nickel affinity chromatography, to over 95% homogeneity

Pyrobaculum calidifontis

A3MSP1

758887

recombinant His6-tagged enzyme from Escherichia coli by heat treatment for 15 min at 65°C, nickel affinity chromatography, ultrafiltration, and dialysis, the tag is cleaved off by Factor Xa, followed by another step of nickel affinity chromatography

Pyrobaculum calidifontis

A3MSP1

743764