2.6.1.B18: archeosine synthase (ammonia)
This is an abbreviated version!
For detailed information about archeosine synthase (ammonia), go to the full flat file.
Reaction
Synonyms
amidinotransferase QueF-Like, ammonium:preQ0-tRNA aminotransferase, Pcal_0221, QueF-L, QueF-like
ECTree
2.6.1.B18 archeosine synthase (ammonia)Advanced search results
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results (Crystallization
Crystallization on EC 2.6.1.B18 - archeosine synthase (ammonia)
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CRYSTALLIZATION (Commentary) 
ORGANISM
UNIPROT
LITERATURE
purified recombinant wild-type or SeMet-labeled enzyme in complex with preQ0, hanging drop vapour diffusion method, mixing of 0.001 ml of protein solution with 0.001 ml of reservoir solution containing 17-19% PEG 3350, 0.15 M potassium thiocyanate, and 0.05% sodium azide, and equilibration against 0.5 mL of reservoir solution, for the SeMet-labeled protein in complex with preQ0, the same conditions are used, except crystallization is conducted in sitting drops set up by mixing 0.002 ml of protein solution with 0.001 ml of reservoir solution, 20°C, 1-2 weeks, X-ray diffraction structure determination and analysis at 1.91 A and 2.74 A resolution, respectively, modelling
structure in the presence of preQ0, reveals a symmetric T-fold homodecamer of two head-to-head facing pentameric subunits, with 10 active sites at the inter-monomer interfaces. Bound preQ0 forms a stable covalent thioimide bond with a conserved active site cysteine. QueF-L lacks a NADPH binding site
