1.14.14.90: isoflavone 2'-hydroxylase
This is an abbreviated version!
For detailed information about isoflavone 2'-hydroxylase, go to the full flat file.
Word Map on EC 1.14.14.90
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1.14.14.90
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isoflavonoids
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phytoalexins
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glycyrrhiza
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flavonoid
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echinata
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genistein
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cinnamic
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4-hydroxylase
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phenylpropanoids
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chickpea
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medicarpin
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cicer
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elicitor
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legume
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licorice
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2'-hydroxylated
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arietinum
- 1.14.14.90
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isoflavonoids
-
phytoalexins
- glycyrrhiza
- flavonoid
- echinata
- genistein
-
cinnamic
-
4-hydroxylase
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phenylpropanoids
- chickpea
- medicarpin
-
cicer
- elicitor
-
legume
- licorice
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2'-hydroxylated
- arietinum
Reaction
Synonyms
AmI2'H, CYP Ge-3, CYP81E, CYP81E1, CYP81E11, CYP81E12, CYP81E13, CYP81E42, EC 1.14.13.89, I2'H, isoflavone 2'-monooxygenase, LjCY-2 protein, MtCYP81E7
ECTree
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Substrates Products
Substrates Products on EC 1.14.14.90 - isoflavone 2'-hydroxylase
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REACTION DIAGRAM
3'-hydroxyformononetin + [reduced NADPH-hemoprotein reductase] + O2
2',3'-dihydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O
4.7% of the activity with biochanin A
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-
?
biochanin A + [reduced NADPH-hemoprotein reductase] + O2
2'-hydroxybiochanin A + [oxidized NADPH-hemoprotein reductase] + H2O
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-
-
?
daidzein + [reduced NADPH-hemoprotein reductase] + O2
2'-hydroxydaidzein + [oxidized NADPH-hemoprotein reductase] + H2O
formononetin + [reduced NADPH-hemoprotein reductase] + O2
2'-hydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O
genistein + [reduced NADPH-hemoprotein reductase] + O2
2'-hydroxygenistein + [oxidized NADPH-hemoprotein reductase] + H2O
2'-hydroxydaidzein + [oxidized NADPH-hemoprotein reductase] + H2O
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-
-
?
daidzein + [reduced NADPH-hemoprotein reductase] + O2
2'-hydroxydaidzein + [oxidized NADPH-hemoprotein reductase] + H2O
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-
-
?
daidzein + [reduced NADPH-hemoprotein reductase] + O2
2'-hydroxydaidzein + [oxidized NADPH-hemoprotein reductase] + H2O
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-
-
?
daidzein + [reduced NADPH-hemoprotein reductase] + O2
2'-hydroxydaidzein + [oxidized NADPH-hemoprotein reductase] + H2O
18.6% of the activity with biochanin A
+ low concentrations of 3'-hydroxydaidzein
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?
2'-hydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O
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-
-
?
formononetin + [reduced NADPH-hemoprotein reductase] + O2
2'-hydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O
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-
-
?
formononetin + [reduced NADPH-hemoprotein reductase] + O2
2'-hydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O
37.2% of the activity with biochanin A
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-
?
2'-hydroxygenistein + [oxidized NADPH-hemoprotein reductase] + H2O
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-
-
?
genistein + [reduced NADPH-hemoprotein reductase] + O2
2'-hydroxygenistein + [oxidized NADPH-hemoprotein reductase] + H2O
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-
-
?
genistein + [reduced NADPH-hemoprotein reductase] + O2
2'-hydroxygenistein + [oxidized NADPH-hemoprotein reductase] + H2O
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-
-
?
genistein + [reduced NADPH-hemoprotein reductase] + O2
2'-hydroxygenistein + [oxidized NADPH-hemoprotein reductase] + H2O
10.2% of the activity with biochanin A
+ low concentrations of 3'-hydroxygenistaien
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?
?
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no activity with formononetin, cf. EC 1.14.13.53, 4'-methoxyisoflavone 2'-hydroxylase
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-
?
additional information
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no activity with formononetin, cf. EC 1.14.13.53, 4'-methoxyisoflavone 2'-hydroxylase
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?
additional information
?
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the enzyme is involved in biosynthesis of flavonoids
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-
?
additional information
?
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the enzyme is involved in biosynthesis of flavonoids
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-
?
additional information
?
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the enzyme is involved in the biosynthesis of isoflavonoid-derived antimicrobial compounds of legumes
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-
?
additional information
?
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no activity with naringenin (5,7,4'-trihydroxyflavanone), liquiritigenin (7,4'-dihydroxyflavanone), trans-cinnamic acid and 4-coumaric acid
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-
?
additional information
?
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the enzyme is involved in biosynthesis of flavonoids
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-
?
additional information
?
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the enzyme is involved in the biosynthesis of isoflavonoid-derived antimicrobial compounds of legumes
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-
?
additional information
?
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no activity with naringenin (5,7,4'-trihydroxyflavanone), liquiritigenin (7,4'-dihydroxyflavanone), trans-cinnamic acid and 4-coumaric acid
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-
?
additional information
?
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the enzyme is essential for isoflavan pterocarpan phytoalexin biosynthesis
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-
?
additional information
?
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no activity with isoformononetin, prunetin, 6,7,4'-trihydroxyisoflavone, 3',4',7-trihydroxyisoflavone
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?
additional information
?
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no activity with isoformononetin, prunetin, 6,7,4'-trihydroxyisoflavone, 3',4',7-trihydroxyisoflavone
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-
?