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1.14.14.90: isoflavone 2'-hydroxylase

This is an abbreviated version!
For detailed information about isoflavone 2'-hydroxylase, go to the full flat file.

Word Map on EC 1.14.14.90

Reaction

an isoflavone
+
[reduced NADPH-hemoprotein reductase]
 +
O2
=
a 2'-hydroxyisoflavone
 +
[oxidized NADPH-hemoprotein reductase]
 +
H2O

Synonyms

AmI2'H, CYP Ge-3, CYP81E, CYP81E1, CYP81E11, CYP81E12, CYP81E13, CYP81E42, EC 1.14.13.89, I2'H, isoflavone 2'-monooxygenase, LjCY-2 protein, MtCYP81E7

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.14.90 isoflavone 2'-hydroxylase

Cloned

Cloned on EC 1.14.14.90 - isoflavone 2'-hydroxylase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
dual plasmid method development for functional expression of plant CYPs in Escherichia coli, method and culture conditions optimization, overview. Gene CYP81E11, DNA and amino acid sequence determination and analysis, recombinant expression of the transmembrane-domain truncated CYP enzyme in Escherichia coli strain C41(DE3) and coexpression with CPR from Lotus japonicus as a discrete polypeptide. The optimal temperature is 25°C, addition of the heme precursor 5-aminolevulinic acid is essential for functional expression of CYP81E11
dual plasmid method development for functional expression of plant CYPs in Escherichia coli, method and culture conditions optimiztaion, overview. Gene CYP81E12, DNA and amino acid sequence determination and analysis, recombinant expression of the transmembrane-domain truncated CYP enzyme in Escherichia coli strain C41(DE3) and coexpression with CPR from Lotus japonicus as a discrete polypeptide. The optimal temperature is 25°C, addition of the heme precursor 5-aminolevulinic acid is essential for functional expression of CYP81E12
dual plasmid method development for functional expression of plant CYPs in Escherichia coli, method and culture conditions optimiztaion, overview. Gene CYP81E13, DNA and amino acid sequence determination and analysis, recombinant expression of the transmembrane-domain truncated CYP enzyme in Escherichia coli strain C41(DE3) and coexpression with CPR from Lotus japonicus as a discrete polypeptide. The optimal temperature is 25°C, addition of the heme precursor 5-aminolevulinic acid is essential for functional expression of CYP81E13
expression in Saccharomyces cerevisiae
expression in yeast
-
expression in yeast strain WAT11
gene AmI2'H or CYP81E42, DNA and amino acid sequence determination and analysis, recombinant expression of truncated enzyme, lacking the N-terminal membrane binding motif, in Escherichia coli strain BL21(DE3). Expression of the enzyme without removal of the transmembrane domain or insertion of the hydrophilic peptide AKKTSSKGKL is unsuccessful