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0.000011
-
pulmonary IDO activity in pneumothorax patients
0.000014
-
pulmonary IDO activity in benign tumor patient
0.000015
-
pulmonary IDO activity in tuberculosis patient
0.000039
-
pulmonary IDO activity in bronchiectasis patient
0.00005
-
pulmonary IDO activity in lung cancer patients
0.000067
-
pulmonary IDO activity in pulmonary abscess patient
0.000192
-
L-tryptophan, Tris-buffer, pH 8.0, 0.0001 mM methylene blue and 0.001 mM each of ATP and Mg2+, 37°C, 90 min, no rat-pretreatment
0.000208
-
D-tryptophan, Tris-buffer, pH 8.0, 0.0001 mM methylene blue and 0.001 mM each of ATP and Mg2+, 37°C, 90 min, no rat-pretreatment
0.000224
-
D-tryptophan, Tris-buffer, pH 8.0, 0.0001 mM methylene blue and 0.001 mM each of ATP and Mg2+, 37°C, 90 min, rat-pretreatment with neomycin
0.000245
-
L-tryptophan, Tris-buffer, pH 8.0, 0.0001 mM methylene blue and 0.001 mM each of ATP and Mg2+, 37°C, 90 min, rat-pretreatment with cortisone
0.000256
-
D-tryptophan, Tris-buffer, pH 8.0, 0.0001 mM methylene blue and 0.001 mM each of ATP and Mg2+, 37°C, 90 min, rat-pretreatment with cortisone
0.000272
-
L-tryptophan, Tris-buffer, pH 8.0, 0.0001 mM methylene blue and 0.001 mM each of ATP and Mg2+, 37°C, 90 min, rat-pretreatment with D-tryptophan
0.000277
-
D-tryptophan, Tris-buffer, pH 8.0, 0.0001 mM methylene blue and 0.001 mM each of ATP and Mg2+, 37°C, 90 min, rat-pretreatment with D-tryptophan
0.00033
-
crude extract of human lung slices treated with gamma-IFN
0.000598
-
supernatant, 0.8 mM L-tryptophan, 40 mM ascorbic acid, 0.02 mM methylene blue, 200 units/ml catalase, 100 mM potassium phosphate buffer, pH 6.5, 37°C, 30 min
0.011
-
brain, in air, 50 mM potassium phosphate buffer, pH 6.5, 0.025 mM methylene blue, 20 mM ascorbate, 0.05 mM catalase, 0.4 mM L-tryptophan, 37°C
0.014
-
brain, in 1.2 atm oxygen, 50 mM potassium phosphate buffer, pH 6.5, 0.025 mM methylene blue, 20 mM ascorbate, 0.05 mM catalase, 0.4 mM L-tryptophan, 37°C
0.022
-
sample 14 of 22 cervical mucus sampples, determined by HPLC/UV detection, reaction mixture include 0.025 mM methylene blue, 20 mM ascorbic acid, 0.04 mM L-tryptophan solution, 0.05 ml catalase and 50 mM potassium phosphate buffer (pH 6.5), 30 min, 37°C
0.024
-
sample 17 of 22 cervical mucus sampples, determined by HPLC/UV detection, reaction mixture include 0.025 mM methylene blue, 20 mM ascorbic acid, 0.04 mM L-tryptophan solution, 0.05 ml catalase and 50 mM potassium phosphate buffer (pH 6.5), 30 min, 37°C
0.04
-
small intestine IV, 0.05 mM potassium phosphate buffer (pH 7.5), 0.005 mM methylene blue, 0.01 mM ascorbic acid, 0.003 mM tryptophan, 37°C
0.05
-
small intestine proximal portion, 0.05 mM potassium phosphate buffer (pH 7.5), 0.005 mM methylene blue, 0.01 mM ascorbic acid, 0.003 mM tryptophan, 37°C
0.072
-
assuming an average wet weight of the lens of 0.2 g, assay in 50 mM potassium phosphate buffer (pH 6.5), 20 mM ascorbic acid, 0.01 mM methylene blue, 0.1 mg/ml catalase, 0.4 mM L-tryptophan, 90 min, 37°C
0.08
-
appendix, 0.05 mM potassium phosphate buffer (pH 7.5), 0.005 mM methylene blue, 0.01 mM ascorbic acid, 0.003 mM tryptophan, 37°C
0.122
-
submandibular gland
0.145
-
small intestine, in 5.2 atm oxygen, 50 mM potassium phosphate buffer, pH 6.5, 0.025 mM methylene blue, 20 mM ascorbate, 0.05 mM catalase, 0.4 mM L-tryptophan, 37°C
0.15
-
appendix, 0.05 mM potassium phosphate buffer (pH 7.5), 0.005 mM methylene blue, 0.01 mM ascorbic acid, 0.003 mM tryptophan, 37°C
0.198
-
lung, in air, 50 mM potassium phosphate buffer, pH 6.5, 0.025 mM methylene blue, 20 mM ascorbate, 0.05 mM catalase, 0.4 mM L-tryptophan, 37°C
0.232
-
brain, lipopolysaccharide-treated mice
0.278
-
esophagus, lipopolysaccharide-treated mice
0.342
-
submandibular gland, lipopolysaccharide-treated mice
0.474
-
sample 11 of 22 cervical mucus sampples, determined by HPLC/UV detection, reaction mixture include 0.025 mM methylene blue, 20 mM ascorbic acid, 0.04 mM L-tryptophan solution, 0.05 ml catalase and 50 mM potassium phosphate buffer (pH 6.5), 30 min, 37°C
0.479
-
sample 19 of 22 cervical mucus sampples, determined by HPLC/UV detection, reaction mixture include 0.025 mM methylene blue, 20 mM ascorbic acid, 0.04 mM L-tryptophan solution, 0.05 ml catalase and 50 mM potassium phosphate buffer (pH 6.5), 30 min, 37°C
0.55
-
25°C, 50 mM HEPES/NaOH (pH 7.3), 5 mM D-tryptophan 0.01 mM methylene blue, 5 mM ascorbic acid, 0.001 mM catalase
0.56
-
testis, lipopolysaccharide-treated mice
0.57
-
heart, lipopolysaccharide-treated mice
0.636
-
sample 15 of 22 cervical mucus sampples, determined by HPLC/UV detection, reaction mixture include 0.025 mM methylene blue, 20 mM ascorbic acid, 0.04 mM L-tryptophan solution, 0.05 ml catalase and 50 mM potassium phosphate buffer (pH 6.5), 30 min, 37°C
0.689
-
sample 21 of 22 cervical mucus sampples, determined by HPLC/UV detection, reaction mixture include 0.025 mM methylene blue, 20 mM ascorbic acid, 0.04 mM L-tryptophan solution, 0.05 ml catalase and 50 mM potassium phosphate buffer (pH 6.5), 30 min, 37°C
0.735
-
trachea, lipopolysaccharide-treated mice
0.755
-
spleen, lipopolysaccharide-treated mice
0.8
-
thymus, lipopolysaccharide-treated mice
0.873
-
lung, in 5.2 atm oxygen, 50 mM potassium phosphate buffer, pH 6.5, 0.025 mM methylene blue, 20 mM ascorbate, 0.05 mM catalase, 0.4 mM L-tryptophan, 37°C
0.905
-
lung, in 1.2 atm oxygen, 50 mM potassium phosphate buffer, pH 6.5, 0.025 mM methylene blue, 20 mM ascorbate, 0.05 mM catalase, 0.4 mM L-tryptophan, 37°C
0.913
-
sample 13 of 22 cervical mucus sampples, determined by HPLC/UV detection, reaction mixture include 0.025 mM methylene blue, 20 mM ascorbic acid, 0.04 mM L-tryptophan solution, 0.05 ml catalase and 50 mM potassium phosphate buffer (pH 6.5), 30 min, 37°C
1.39
-
urinary bladder, lipopolysaccharide-treated mice
1.902
-
stomach, lipopolysaccharide-treated mice
104.9
-
epididymis, lipopolysaccharide-treated mice
12.5
-
cells grown on L-alanine
15.31
-
cecum, lipopolysaccharide-treated mice
15.6
-
cells grown on L-asparagine
2.13
-
25°C, 50 mM HEPES/NaOH (pH 7.3), 2 mM L-tryptophan 0.01 mM methylene blue, 5 mM ascorbic acid, 0.001 mM catalase
2.48
-
kynurenine, 50 mM potassium phosphate buffer (pH 6.5), 20 mM ascorbic acid, 0.2 mg/ml catalase, 0.01 mM methylene blue, 0.4 mM tryptophan, 37°C, 10-60 min
22.7
-
cells grown on L-tryptophan
23.5
-
cells grown on L-arginine
234
-
enzyme after purification, 0.05 mM potassium phosphate buffer (pH 7.5), 0.005 mM methylene blue, 0.01 mM ascorbic acid, 0.003 mM tryptophan, 37°C
26.68
-
colon, lipopolysaccharide-treated mice
4.07
-
intestine, lipopolysaccharide-treated mice
5.19
-
small intestine VI, 0.05 mM potassium phosphate buffer (pH 7.5), 0.005 mM methylene blue, 0.01 mM ascorbic acid, 0.003 mM tryptophan, 37°C
5.34
-
lung, lipopolysaccharide-treated mice
6.97
-
small intestine distal portion, 0.05 mM potassium phosphate buffer (pH 7.5), 0.005 mM methylene blue, 0.01 mM ascorbic acid, 0.003 mM tryptophan, 37°C
8.33
-
seminal vesicle, lipopolysaccharide-treated mice
0.01
-
ascending colon, 0.05 mM potassium phosphate buffer (pH 7.5), 0.005 mM methylene blue, 0.01 mM ascorbic acid, 0.003 mM tryptophan, 37°C
0.01
-
caecum, 0.05 mM potassium phosphate buffer (pH 7.5), 0.005 mM methylene blue, 0.01 mM ascorbic acid, 0.003 mM tryptophan, 37°C
0.07
-
brain, in 5.2 atm oxygen, 50 mM potassium phosphate buffer, pH 6.5, 0.025 mM methylene blue, 20 mM ascorbate, 0.05 mM catalase, 0.4 mM L-tryptophan, 37°C
0.07
-
small intestine V, 0.05 mM potassium phosphate buffer (pH 7.5), 0.005 mM methylene blue, 0.01 mM ascorbic acid, 0.003 mM tryptophan, 37°C
0.24
-
kidney, lipopolysaccharide-treated mice
0.24
-
small intestine, in 1.2 atm oxygen, 50 mM potassium phosphate buffer, pH 6.5, 0.025 mM methylene blue, 20 mM ascorbate, 0.05 mM catalase, 0.4 mM L-tryptophan, 37°C
0.38
-
0.38
-
small intestine, in air, 50 mM potassium phosphate buffer, pH 6.5, 0.025 mM methylene blue, 20 mM ascorbate, 0.05 mM catalase, 0.4 mM L-tryptophan, 37°C
2.67
-
-
2.67
-
kynurenine, 50 mM potassium phosphate buffer (pH 6.5), 20 mM ascorbic acid, 0.2 mg/ml catalase, 0.01 mM methylene blue, 0.4 mM tryptophan, 37°C, 60 min
additional information
-
-
additional information
low IDO activity of MIP protein, no activity by IDO-like Mb protein
additional information
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0.0142 nmol/mg/lens, assay in 50 mM potassium phosphate buffer (pH 6.5), 20 mM ascorbic acid, 0.01 mM methylene blue, 0.1 mg/ml catalase, 0.4 mM L-tryptophan, 90 min, 37°C
additional information
-
-
additional information
-
poor activity in cells grown on arginine and on glucose, also in presence of L-tryptophan
additional information
-
-
additional information
-
-
additional information
-
-
additional information
-
influence of some methodological factors on measurement of tryptophan oxygenase activities in crude homogenates of rat liver