Inhibitors | Comment | Organism | Structure |
---|---|---|---|
H2O2 | inactivated by H2O formed by ascorbic acid in presence of methylen blue | Oryctolagus cuniculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
5-hydroxy-L-tryptophan | 24°C | Oryctolagus cuniculus | |
0.045 | - |
L-Trp | 24°C | Oryctolagus cuniculus | |
0.075 | - |
tryptamine | 24°C | Oryctolagus cuniculus | |
0.1 | - |
serotonin | 24°C | Oryctolagus cuniculus | |
0.4 | - |
D-Trp | 24°C | Oryctolagus cuniculus | |
0.6 | - |
5-hydroxy-D-tryptophan | 24°C | Oryctolagus cuniculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
1 * 40000, SDS-PAGE | Oryctolagus cuniculus |
41000 | - |
1 * 41000, sedimentation velocity experiments in presence of 6 M guanidine HCl | Oryctolagus cuniculus |
42000 | - |
sedimentation velocity experiments | Oryctolagus cuniculus |
54000 | - |
gel filtration | Oryctolagus cuniculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | 5.4% carbohydrate | Oryctolagus cuniculus |
glycoprotein | 1.2 galactose, 2.6 mannose, 5.2 N-acetylglucosamine and 0.8 sialic acid residues per mol of enzyme | Oryctolagus cuniculus |
Purification (Comment) | Organism |
---|---|
- |
Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
small intestine | - |
Oryctolagus cuniculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.85 | - |
- |
Oryctolagus cuniculus |
Storage Stability | Organism |
---|---|
-70°C, stable for at least 2 months | Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-5-hydroxytryptophan + O2 | - |
Oryctolagus cuniculus | ? | - |
? | |
D-Trp + O2 | - |
Oryctolagus cuniculus | N-formyl-D-kynurenine | - |
? | |
L-5-hydroxytryptophan + O2 | - |
Oryctolagus cuniculus | ? | - |
? | |
L-Trp + O2 | - |
Oryctolagus cuniculus | L-formylkynurenine | - |
? | |
additional information | in the presence of guaiacol or potassium iodide the enzyme degrades H2O2 | Oryctolagus cuniculus | ? | - |
? | |
serotonin + O2 | - |
Oryctolagus cuniculus | ? | - |
? | |
tryptamine + O2 | - |
Oryctolagus cuniculus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 40000, SDS-PAGE | Oryctolagus cuniculus |
monomer | 1 * 41000, sedimentation velocity experiments in presence of 6 M guanidine HCl | Oryctolagus cuniculus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
pH 6.0, 5 min, 43% loss of dioxygenase activity on L-Trp and D-Trp, 55% loss ofter 10 min, 70% loss ofter 20 min | Oryctolagus cuniculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00283 | - |
serotonin | 24°C | Oryctolagus cuniculus | |
0.00483 | - |
tryptamine | 24°C | Oryctolagus cuniculus | |
0.00967 | - |
5-hydroxy-D-tryptophan | 24°C | Oryctolagus cuniculus | |
0.0433 | - |
5-hydroxy-L-tryptophan | 24°C | Oryctolagus cuniculus | |
1.18 | - |
D-Trp | 24°C | Oryctolagus cuniculus | |
1.65 | - |
L-Trp | - |
Oryctolagus cuniculus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | - |
4°C, 6 h, 52% loss of activity | Oryctolagus cuniculus |
5 | - |
4°C, 6 h, 7% loss of activity | Oryctolagus cuniculus |
6 | - |
4°C, no loss of activity after 6 h | Oryctolagus cuniculus |
7 | - |
4°C, 6 h, 15% loss of activity | Oryctolagus cuniculus |
8 | - |
4°C, 6 h, 55% loss of activity | Oryctolagus cuniculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | 0.8 mol of protoheme IX per mol of enzyme | Oryctolagus cuniculus |