EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
---|
2.4.1.25 | 4 |
- |
quite stable at low temperatures |
488995 |
2.4.1.25 | 35 |
40 |
at short incubation time for 15 min at 40°C, the remaining activity of the wild type enzyme is 39.5%, whereas for 30 min incubation, the activity remained is 15.2%. At 35°C for a longer incubation time of 3 h, the remaining activity of the wild type enzyme is 45% |
736255 |
2.4.1.25 | 35 |
45 |
the specific activity remaining after 10 min at 35°C for the wild type enzyme is 49.3% while that for mutant enzyme N287Y is 91.4%. At 40°C, no activity of the wild type enzyme is left after 10 min but mutant N287Y shows 34.3% remaining activity. At 45°C, the wild type loses all activity after 10 min incubation time while 28.6% of the activity of mutant N287Y still remains |
755942 |
2.4.1.25 | 37 |
42 |
D-enzyme, activity decreases rapidly above 37°C and is almost completely lost at 42°C |
488998 |
2.4.1.25 | 37 |
80 |
stable at 37°C, retains more than 90% of its maximum activity between 55-80°C, half-life of activity of about 3 h at 80°C |
489005 |
2.4.1.25 | 45 |
- |
stable up to |
489002 |
2.4.1.25 | 50 |
- |
10 min, almost complete loss of activity |
718654 |
2.4.1.25 | 50 |
- |
amylomaltase, rapidly inactivated at temperatures above |
488998 |
2.4.1.25 | 50 |
80 |
the recombinant enzyme maintains more than 80% of its activity at a temperature range of 50-80°C and a pH range of 5.0-9.0 |
704054 |
2.4.1.25 | 51.5 |
- |
melting temperature |
756915 |