Literature summary for 2.4.1.25 extracted from

Nimpiboon, P.; Krusong, K.; Kaulpiboon, J.; Kidokoro, S.; Pongsawasdi, P.
Roles of N287 in catalysis and product formation of amylomaltase from Corynebacterium glutamicum (2016), Biochem. Biophys. Res. Commun., 478, 759-764 .

Search for more literature for 2.4.1.25

Protein Variants

Protein Variants	Comment	Organism
N287Y	the mutant shows a significant decrease in all transglucosylation activities including starch transglucosylation, disproportionation, cyclization and coupling compared to the wild type enzyme, while hydrolysis activity is not changed. The mutant shows an increase in thermostability and substrate preference for maltoheptaose in addition to maltotriose	Corynebacterium glutamicum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
14.5	-	maltotriose	mutant enzyme N287Y, at pH 6.0 and 30°C	Corynebacterium glutamicum
15.4	-	maltopentaose	mutant enzyme N287Y, at pH 6.0 and 30°C	Corynebacterium glutamicum
15.5	-	maltotriose	wild type enzyme, at pH 6.0 and 30°C	Corynebacterium glutamicum
18.6	-	maltopentaose	wild type enzyme, at pH 6.0 and 30°C	Corynebacterium glutamicum

Organism

Organism	UniProt	Comment	Textmining
Corynebacterium glutamicum	-	-	-
Corynebacterium glutamicum ATCC 13032	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
HisTrap affinity column chromatography	Corynebacterium glutamicum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
maltohexaose + glycosyl acceptor	-	Corynebacterium glutamicum	maltose + D-glucose + maltooligosaccharides	-	?
maltohexaose + glycosyl acceptor	-	Corynebacterium glutamicum ATCC 13032	maltose + D-glucose + maltooligosaccharides	-	?
maltopentaose + glycosyl acceptor	-	Corynebacterium glutamicum	maltose + D-glucose + maltooligosaccharides	-	?
maltopentaose + glycosyl acceptor	-	Corynebacterium glutamicum ATCC 13032	maltose + D-glucose + maltooligosaccharides	-	?
maltose + glycosyl acceptor	lowest activity	Corynebacterium glutamicum	?	-	?
maltose + glycosyl acceptor	lowest activity	Corynebacterium glutamicum ATCC 13032	?	-	?
maltotetraose + glycosyl acceptor	second best substrate	Corynebacterium glutamicum	maltose + D-glucose + maltooligosaccharides	-	?
maltotetraose + glycosyl acceptor	second best substrate	Corynebacterium glutamicum ATCC 13032	maltose + D-glucose + maltooligosaccharides	-	?
maltotriose + glycosyl acceptor	best substrate	Corynebacterium glutamicum	maltose + D-glucose + maltooligosaccharides	-	?
maltotriose + glycosyl acceptor	best substrate	Corynebacterium glutamicum ATCC 13032	maltose + D-glucose + maltooligosaccharides	-	?
pea starch + glycosyl acceptor	-	Corynebacterium glutamicum	large ring-cyclodextrins 23-26	-	?

Synonyms

Synonyms	Comment	Organism
amylomaltase	-	Corynebacterium glutamicum

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	optimum activity for cyclization activity	Corynebacterium glutamicum
45	-	optimum activity for disproportionation reaction	Corynebacterium glutamicum

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
35	45	the specific activity remaining after 10 min at 35°C for the wild type enzyme is 49.3% while that for mutant enzyme N287Y is 91.4%. At 40°C, no activity of the wild type enzyme is left after 10 min but mutant N287Y shows 34.3% remaining activity. At 45°C, the wild type loses all activity after 10 min incubation time while 28.6% of the activity of mutant N287Y still remains	Corynebacterium glutamicum

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.025	-	maltopentaose	mutant enzyme N287Y, at pH 6.0 and 30°C	Corynebacterium glutamicum
0.033	-	maltotriose	mutant enzyme N287Y, at pH 6.0 and 30°C	Corynebacterium glutamicum
4.8	-	maltopentaose	wild type enzyme, at pH 6.0 and 30°C	Corynebacterium glutamicum
10.4	-	maltotriose	wild type enzyme, at pH 6.0 and 30°C	Corynebacterium glutamicum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	-	Corynebacterium glutamicum

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.0017	-	maltopentaose	mutant enzyme N287Y, at pH 6.0 and 30°C	Corynebacterium glutamicum
0.0023	-	maltotriose	mutant enzyme N287Y, at pH 6.0 and 30°C	Corynebacterium glutamicum
0.26	-	maltopentaose	wild type enzyme, at pH 6.0 and 30°C	Corynebacterium glutamicum
0.68	-	maltotriose	wild type enzyme, at pH 6.0 and 30°C	Corynebacterium glutamicum

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Release 2023.1 (January 2023)