Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 Star (DE3) cells | Culex quinquefasciatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | toxic BinA component of the activated binary toxin does not inhibit alpha-glucosidase activity of the enzyme, while BinB reduces the activity by nearly 50% | Culex quinquefasciatus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | Ca2+ provides structural stability to the protein | Culex quinquefasciatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
120000 | - |
gel filtration | Culex quinquefasciatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Culex quinquefasciatus | A0A222C758 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-IDA matrix chromatography | Culex quinquefasciatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltose + glycosyl acceptor | efficient transglycosylation activity | Culex quinquefasciatus | D-glucose | - |
? | |
maltotriose + glycosyl acceptor | efficient transglycosylation activity | Culex quinquefasciatus | D-glucose + maltooligosaccharides | - |
? | |
additional information | the enzyme displays weak hydrolytic activity against sucrose and alpha-aryl glucoside and fails to act on trehalose and isomaltose | Culex quinquefasciatus | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 62329, calculated from amino acid sequence | Culex quinquefasciatus |
homodimer | 2 * 62378, MALDI-TOF mass spectrometry | Culex quinquefasciatus |
Synonyms | Comment | Organism |
---|---|---|
amylomaltase | - |
Culex quinquefasciatus |
Cqm1 | the ordered domain (residues 23-560) of surface receptor protein Cqm1 has amylomaltase activity | Culex quinquefasciatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Culex quinquefasciatus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
51.5 | - |
melting temperature | Culex quinquefasciatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Culex quinquefasciatus |