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EC 1.5.3.24 Details
show Brenda entryEC number
1.5.3.24
Accepted name
sarcosine oxidase (5,10-methylenetetrahydrofolate-forming)
Reaction
sarcosine + 5,6,7,8-tetrahydrofolate + O2 = glycine + 5,10-methylenetetrahydrofolate + H2O2
Other name(s)
TSOX,
sarcosine oxidase (ambigious),
heterotetrameric sarcosine oxidase
Systematic name
sarcosine, 5,6,7,8-tetrahydrofolate:O2 oxidoreductase (demethylating,5,10-methylenetetrahydrofolate-forming)
CAS registry number
9029-22-5
Comment
The enzyme, found in some bacterial species, is composed of four different subunits and two active sites connected by a large "reaction chamber". An imine intermediate is transferred between the sites, eliminating the production of toxic formaldehyde. The enzyme contains three cofactors: noncovalently bound FAD and NAD+, and FMN that is covalently bound to a histidine residue. In the absence of folate the enzyme catalyses the reaction of EC 1.5.3.1, sarcosine oxidase (formaldehyde-forming).
History
created 2022
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1 Oxidoreductases
1.1 Acting on the CH-OH group of donors
1.5.3.3 created 1961, deleted 1972 1.5.3.8 created 1989, deleted 1992 1.5.3.9 created 1989, modified 1999, deleted 2002
