EC Explorer

1.97.1.4

[formate-C-acetyltransferase]-activating enzyme

S-adenosyl-L-methionine + dihydroflavodoxin + [formate C-acetyltransferase]-glycine = 5′-deoxyadenosine + L-methionine + flavodoxin semiquinone + [formate C-acetyltransferase]-glycin-2-yl radical

PFL activase, PFL-glycine:S-adenosyl-L-methionine H transferase (flavodoxin-oxidizing, S-adenosyl-L-methionine-cleaving), formate acetyltransferase activating enzyme, formate acetyltransferase-glycine dihydroflavodoxin:S-adenosyl-L-methionine oxidoreductase (S-adenosyl-L-methionine cleaving), pyruvate formate-lyase activating enzyme, pyruvate formate-lyase 1 activating enzyme

[formate C-acetyltransferase]-glycine dihydroflavodoxin:S-adenosyl-L-methionine oxidoreductase (S-adenosyl-L-methionine cleaving)

206367-15-9

An iron-sulfur protein. A single glycine residue in EC 2.3.1.54, formate C-acetyltransferase, is oxidized to the corresponding radical by transfer of H from its CH₂ to AdoMet with concomitant cleavage of the latter. The reaction requires Fe²⁺. The first stage is reduction of the AdoMet to give methionine and the 5′-deoxyadenosin-5′-yl radical, which then abstracts a hydrogen radical from the glycine residue.

created 1999, modified 2004