2.7.1.71: shikimate kinase

This is an abbreviated version!
For detailed information about shikimate kinase, go to the full flat file.

Reaction

Synonyms

adenosine triphosphate: shikimate-3-phosphotransferase, AroK, AroL, AtSK1, AtSK2, kinase (phosphorylating), shikimate, kinase, shikimate (phosphorylating), MtSK, OsSK1, OsSK2, OsSK3, Rv2539c, shikimate kinase II, shikimate kinase-like 1, SK I, SK II, SK1, SK2, SKI, SKII, SKL1, type I shikimate kinase, aroK-encoded, type II shikimate kinase

ECTree

     2 Transferases

         2.7 Transferring phosphorus-containing groups

             2.7.1 Phosphotransferases with an alcohol group as acceptor

                2.7.1.71 shikimate kinase

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Results	in table
45743	AA Sequence
7	Application
1	CAS Registry Number
19	Cloned(Commentary)
16	Cofactor
20	Crystallization (Commentary)
1	Expression
22	General Information
5	General Stability
14	IC50 Value
185	Inhibitors
8	kcat/KM [mM/s]
88	Ki Value [mM]
44	KM Value [mM]
1	Localization
51	Metals/Ions
28	Molecular Weight [Da]
49	Natural Substrates/ Products (Substrates)
731	Organism
6	Pathway
70	PDB ID
6	pH Optimum
2	pH Range
3	pI Value
1	Posttranslational Modification
33	Protein Variants
20	Purification (Commentary)
3	Reaction
1	Reaction Type
99	Reference
2	Renatured (Commentary)
10	Source Tissue
6	Specific Activity [micromol/min/mg]
5	Storage Stability
101	Substrates and Products (Substrate)
20	Subunits
51	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
2	Temperature Range [°C]
5	Temperature Stability [°C]
15	Turnover Number [1/s]

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RENATURED/Commentary

ORGANISM

UNIPROT

LITERATURE

the enzyme is fully unfolded in 4 M urea. Approximately 95% of the enzyme activity can be recovered on dilution of the urea from 4 to 0.36 M. Refolding occurs in at least four kinetic phases, the slowest of which corresponds with the regain of shikimate binding and enzyme activity

Dickeya chrysanthemi

-

641913

when the enzyme is unfolded by incubation in 4 M urea, addition of NaCl or Na2SO4 leads to relatively slow refolding of the enzyme. The refolded enzyme can bind shikimate, though more weakly than the native enzyme. The refolded enzyme does not appear to be capable of binding nucleotides, nor does it possess detectable catalytic activity. The refolding process brought about by addition of salt in the presence of 4 M urea is not associated with any change in the fluorescence of the probe 8-anilino-1-naphthalenesulfonic acid

Dickeya chrysanthemi

-

641900