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2.7.1.40: pyruvate kinase

This is an abbreviated version!
For detailed information about pyruvate kinase, go to the full flat file.

Word Map on EC 2.7.1.40

Reaction

ATP
+
pyruvate
=
ADP
 +
phosphoenolpyruvate

Synonyms

ATP/pyruvate O'-phosphotransferase, ATP:pyruvate 2-O-phosphotransferase, CPK, CPK1, cPK2, cPK3, cPK4, cPK5, CTHBP, cytosolic pyruvate kinase, cytosolic thyroid hormone binding protein, EHI_098420, EhPK, EhPyk, erythroid (R-type) pyruvate kinase, fluorokinase, hL-PYK, hLPYK, hPKM2, K+-dependent PK, K+-independent PK, kinase, fluoro- (phosphorylating), kinase, pyruvate (phosphorylating), L-PK , L-PYK, liver PK, liver pyruvate kinase, M1-PK, M1-PYK, M2 pyruvate kinase, M2 type phosphoenolpyruvate kinase, M2-PK, M2-pyruvate kinase, M2-type pyruvate kinase, M2PK, mPKM2, MRSA PK, muscle PK, NCgl2008, NCgl2809, PAE0819, PfPYK, pfPyrK, phosphoenol transphosphorylase, phosphoenolpyruvate kinase, phosphoenolpyruvate: ADP phosphotransferase, PK-alphabeta1, PK-alphabeta2, PK-M1, PK-M2, PK-R, PK-S, PK1, PK3, PKC, PKC1, PKL, Pklr, PKM1, PKM2, PKp, ProTalphaK, PYK, PYK-I, Pyk1, Pyk2, PykA, PykF, PyKII, pyruvate kinase, pyruvate kinase 1, pyruvate kinase isoenzyme M2, pyruvate kinase isoform M2, pyruvate kinase isoforms 2, pyruvate kinase isozyme M1, pyruvate kinase isozyme type M2, pyruvate kinase M1, pyruvate kinase M2, pyruvate kinase M2 isoform, pyruvate kinase muscle isoform 2, pyruvate kinase muscle isozyme, pyruvate kinase type M2, pyruvate kinase type-II, pyruvate phosphotransferase, pyruvic kinase, R-type pyruvate kinase, R-type/L-type pyruvate kinase, red cell/liver pyruvate kinase, rM1-PYK, RMPK, RPK, Rv1617, SSO0981, TbrPYK, TCIL3000_10_12020, TcoPYK, THBP1, TuM2-PK, tumor cell specific pyruvate kinase isozyme M2, tumour M2-PK, tumour M2-pyruvate kinase, VcIIPK, VcIPK, VC_0485, VC_2008, VEG17, vegetative protein 17, vesicle-associated pyruvate kinase, YPK

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.40 pyruvate kinase

Purification

Purification on EC 2.7.1.40 - pyruvate kinase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 forms, PK I and II
-
2 immunologically unrelated isoforms, PK1 and 2, separable by anion exchange chromatography
-
2 isozymes, in flight muscle and fat body
-
2 isozymes: type M and type K
-
2 non-interconvertible forms, isozyme PK I: at room temperature
-
aerobic and anoxic variants, i.e. isozymes PK-aerobic and PK-anoxic
Busycotypus canaliculatum
-
affinity chromatography
-
affinity chromatography on Mikacion brilliant yellow 6 GS-Sepharose CL-4B
-
ammonium sulfate precipitation and gel filtration
ammonium sulfate precipitation, DEAE-cellulose column chromatography and Superdex S-200 gel filtration
-
at room temperature, partial
-
Butyl-Sepharose chromatography and Fractogel EMD DEAE-650 (S) anion-exchange chromatography
-
Butyl-Toyopearl 650S chromatography and Resource Q column chromatography
-
Butyl-Toyopearl 650S column chromatography and MonoQ HR10/10 column chromatography
-
DEAE column chromatography and carboxymethyl-Sepharose column chromatography
-
DEAE-Toyo Pearl 650 column chromatography and glutathione Sepharose column chromatography
-
Fractogel EMD SO3- ion exchange chromatography and Fractogel EMD propyl (S) hydrophobic interaction chromatography
-
glutathione Sepharose column chromatography
K4- and M4-isozymes and a hybrid form
-
major of 5 liver isozymes
-
mammalian tissues contain at least 3 distinct and electrophoretically separable kinase isozymes: K-type predominant in fetal tissues, L-type in adult liver and kidney, M-type in brain, skeletal and cardiac muscle
-
multiple isozymes
-
mutant enzyme T298C
-
native cytosolic isozyme 12.3fold from seeds by anion exchange and hydroxyapatite chromatography, inactivation of plastidial isoform by heating the enzyme extract at 60°C for 3 min
-
native enzyme 390fold from skeletal muscle by cation exchange chromatography and affinity chromatography
-
native enzyme from cytosol by ammonium sulfate fractionation and prothymosin alpha affinity and ion exchange chromatography, and chromatography on a phosphocellulose resin
-
native enzyme from liver by two different steps of cation exchange chromatography, 7.3fold
native enzyme from red muscle by cation exchange chromatography, 82.8-97.6fold to homogeneity
Ni-agarose bead chromatography
-
Ni-IDA affinity column chromatography, and gel filtration
-
Ni-NTA column chromatography and DEAE-Sepharose column chromatography
Ni-NTA resin chromatography
-
Ni-NTA resin column chromatography
-
Ni-NTA resinchromatography
-
Ni-Sepharose column chromatography
overview
partial
partial, 2 isoforms, separable by blue-agarose chromatography
-
partial, 3 enzyme types: I, II and III
-
partial, dye-ligand chromatography
-
partial, several isozymes
-
partially purified enzyme preparations from various organisms: overview
posterior adductor muscle
-
recombinant His-tagged enzyme
recombinant His-tagged enzyme from Escherichia coli strain BL21
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, and ultrafiltration, to over 95% purity
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) Rosetta by nickel affinity chromatography, dialysis, and gel filtration
recombinant His-tagged full-length and C-terminally truncated enzymes from Escherichia coli strain BL-21(DE3) by nickel affiniy chromatography
recombinant His-tagged isozyme PK1 from Escherichia coli strain BL21(DE3) by nickel affiniy chromatography
recombinant His-tagged isozyme PK2 from Escherichia coli strain BL21(DE3) by nickel affiniy chromatography
recombinant His-tagged isozyme PKI from Escherichia coli strain BL21-CodonPlus (DE3)-RIL by nickel affinity chromatography and ammonium sulfate fraction, the tag cannot be cleaved, because the cleavage site is not accessible to the TEV protease, to 90% purity
recombinant His-tagged isozyme PKII from Escherichia coli strain BL21-CodonPlus (DE3)-RIL by nickel affinity chromatography and ammonium sulfate fraction, the tag cannot be cleaved, because the cleavage site is not accessible to the TEV protease, to 88% purity
recombinant His-tagged PKM2 from Escherichia coli strain BL21 by nickel affinity chromatography
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3)pLys by nickel affinity chromatography, the His6-tag is cleaved off by PreScission protease followed by a second step of nickel affinity chromatography, ultrafiltration, and gel filtration
recombinant His6-tagged isozymes from Escherichia coli by nickel affinity chromatgraphy
-
recombinant N-terminally His5-tagged enzyme from Escherichia coli by nickel affinity chromatography and desalting gel filtration
recombinant N-terminally His6-tagged enzyme 12.81fold from Escherichia coli strain BL21(DE3) by nickel affinity and ion exchange chromatography
recombinant N-terminally His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 Star (DE3) by nickel affinity chromatography and gel filtration
recombinant untagged isozymes PykA and PykF 2.71fold and 2.1fold, respectively, from Escherichia coli to near homogeneity by anion exchange chromatography and gel filtration
-
recombinant wild-type and mutant enzymes from Escherichia coli strain Bl21(DE3)
recombinant wild-type and mutant enzymes from Escherichia coli strain Rosetta 2 (DE3)pLysS
-
recombinant wild-type and mutant enzymes in Escherichia coli strain BL21
-
recombinant wild-type and mutant H476L from the FF50 strain of Escherichia coli by ammonium sulfate fractionation and anion exchange chromatography
-
recombinnat enzyme from Eschericchia coli strain BL21(DE3) by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration
Sephadex G-100 gel filtration
Sephadex G25 gel filtration, DEAE-cellulose column chromatography, phosphocellulose column chromatography, and ammonium sulfate precipitation
-
two of 5 electrophoretic isoforms: PK1 and PK5
-