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2.7.1.29: glycerone kinase

This is an abbreviated version!
For detailed information about glycerone kinase, go to the full flat file.

Word Map on EC 2.7.1.29

Reaction

ATP
+
Glycerone
=
ADP
 +
glycerone phosphate

Synonyms

acetol kinase, ATP-dependent dihydroxyacetone kinase, dAK, DHA kinase, DhaK, DhaK-2, DhaKI, DhaL, dihydroxyacetone kinase, dihydroxyacetone kinase I, FMN cyclase/dha kinase, glycerone kinase, kinase, acetol (phosphorylating), More, PEP-dependent Dha kinase, phosphoenolpyruvate carbohydrate phosphotransferase

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.29 glycerone kinase

Purification

Purification on EC 2.7.1.29 - glycerone kinase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate, butyl-Toyopearl, UNO-Q6, recombinant enzyme
DEAE-cellulose, ResourceQ, Superdex 200
DEAE-cellulose, Superdex 75
HighQ anion exchange, Superdex 200
HisTrap column chromatography
-
Ni+2/IDA agarose column chromatography and Superdex 75 gel filtration
-
Ni-NTA affinity column chromatography, gel filtration
-
nickel affinity column chromatography and Superdex 200 gel filtration
nickel-iminodiacetic acid agarose column chromatography
polyethylene glycol 6000, polyethyleneimine, DEAE-Sepharose, Sepharose CL-6B
-
streptomycin sulfate, ammonium sulfate, Cibacron blue F3G-ASephadex g-200, DEAE cellulose, partially purified
-