2.5.1.20: rubber cis-polyprenylcistransferase

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For detailed information about rubber cis-polyprenylcistransferase, go to the full flat file.

Word Map on EC 2.5.1.20

2.5.1.20

dolichols

brasiliensis

hevea

allylic

undecaprenyl

parthenium

argentatum

polyprenols

isoprene

prenyltransferases

guayule

polyisoprenoids

cis-1,4-polyisoprene

trans-prenyltransferases

ficus

dimethylallyl

rubber-producing

nogo-b

elastica

neryl

dehydrodolichyl

dhdds

cis-polyisoprene

molecular biology

synthesis

Reaction

a poly-cis-polyprenyl diphosphate longer by one C5 unit

Synonyms

allyltransferase, rubber, chain-elongating enzyme cis-prenyltransferase, cis prenyl transferase, cis-1,4-polyprenylcistransferase, cis-prenyl transferase, cis-prenyltransferase, cisprenyltransferase, CPT, CPT3, CPT6, HRT1, HRT2, isopentenyl pyrophosphate cis-1,4-polyisoprenyl transferase, laticifer-specific rubber-forming enzyme, More, RT-ase complex, rTase, rubber allyltransferase, rubber polymerase, rubber prenyltransferase, rubber transferase, rubber transferase 1, rubber transferase 2, rubber transferase complex, RubCPT1, TkCPT1, TkCPT2, TkCPT3, TkCPTL1

ECTree

2 Transferases

2.5 Transferring alkyl or aryl groups, other than methyl groups

2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)

2.5.1.20 rubber cis-polyprenylcistransferase

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Results	in table
19	Activating Compound
12	AA Sequence
3	Application
1	CAS Registry Number
6	Cloned(Commentary)
2	Cofactor
9	Expression
18	General Information
26	Inhibitors
8	Ki Value [mM]
18	KM Value [mM]
25	Localization
45	Metals/Ions
10	Molecular Weight [Da]
16	Natural Substrates/ Products (Substrates)
67	Organism
1	Oxidation Stability
2	Pathway
1	pH Optimum
1	pH Stability
2	Protein Variants
10	Purification (Commentary)
1	Reaction
1	Reaction Type
62	Reference
45	Source Tissue
4	Specific Activity [micromol/min/mg]
1	Storage Stability
77	Substrates and Products (Substrate)
7	Subunits
71	Synonyms
1	Systematic Name
14	Temperature Optimum [°C]
3	Temperature Stability [°C]

Activating Compound

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Activating Compound on EC 2.5.1.20 - rubber cis-polyprenylcistransferase

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2-mercaptoethanol

Hevea brasiliensis

10 mM, not as effective as dithiothreitol

638726

dithiothreitol

Hevea brasiliensis

10 mM, required for maximal stability

638726

EDTA

Ficus carica

approx. 3.5fold activation at 20 mM

glutathione

neryl diphosphate

accelerates activity

Rubber elongation factor

2 entries

rubber particle

Parthenium argentatum

638730

sulfhydryl compound

Hevea brasiliensis

essential for full activity

638725

additional information

Hevea brasiliensis

recombinant HRT2, expressed in Escherichia coli, is significantly activated in the presence of a centrifuged latex fraction, resulting in the formation of polyisoprenes corresponding to natural rubber, but the recombinant HRT2 does not exhibit significant activity independently. HRT1/HRT2 require additional cofactors from the eukaryotic cells to produce distinct cPT activity, which seem to be contained in latex species

723380