2.4.1.8: maltose phosphorylase
This is an abbreviated version!
For detailed information about maltose phosphorylase, go to the full flat file.
Word Map on EC 2.4.1.8
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2.4.1.8
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sanfranciscensis
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kojibiose
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biotechnology
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beta-glucose
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beta-phosphoglucomutase
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beta2m-free
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mutarotase
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beta2m
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synthesis
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analysis
- 2.4.1.8
- sanfranciscensis
- kojibiose
- biotechnology
- beta-glucose
- beta-phosphoglucomutase
-
beta2m-free
- mutarotase
-
beta2m
- synthesis
- analysis
Reaction
Synonyms
Bsel2056, EoMP4038, GH65 maltose phosphorylase, inverting maltose phosphorylase, MalE, MalP, More, mpA, MPase, phosphorylase, maltose
ECTree
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Engineering
Engineering on EC 2.4.1.8 - maltose phosphorylase
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E487A
mutant loses both the phosphorolytic and the synthetic activity
E487Q
mutant loses the phosphorolytic activity, but retains the synthetic activity (13% of the activity of wild-type MapA)
S634V
mutant loses both the phosphorolytic and the synthetic activity
S635V
mutant loses both the phosphorolytic and the synthetic activity
Y351F
mutant loses both the phosphorolytic and the synthetic activity
E487Q
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mutant loses the phosphorolytic activity, but retains the synthetic activity (13% of the activity of wild-type MapA)
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additional information
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enzymatic synthesis of alpha-anomer-selective D-glucosides using maltose phosphorylase
additional information
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development of a conductometric specific phosphate biosensor based on tri-layer maltose phosphorylase composite films, recombinant enzyme from Escherichia coli is immobilized on a planar interdigitated electrode by cross-linking with saturated glutaraldehyde vapour in the presence of bovine serum albumin, method development and evaluation, overview
additional information
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Replacing maltose hydrolysis with phosphorolysis, by co-expression of the maltose phosphorylase with MAL11 maltose-transporter gene and Lactococcus lactis beta-phosphoglucomutase, in a Saccharomyces cerevisiae straain IMK289, a maltose transporter and maltase knockout strain, increases the anaerobic biomass yield on maltose in anaerobic maltose-limited chemostat cultures by 26%, thus demonstrating the potential of phosphorolysis to improve the free-energy conservation of disaccharide metabolism in industrial microorganisms, phenotype, overview
additional information
the (alpha/alpha)6-barrel loop 3 is a target for specificity engineering to achieve formation of trehalose and kojibiose by the enzyme in yields superior of maltose by reverse phosphorolysis with (alpha1, alpha1)- and alpha-(1,2)-regioselectivity, respectively. Substitution of LaMP His413-Glu421, His413-Ile418 and His413-Glu415 from loop 3, that include His413 and Glu415 presumably recognising the alpha-anomeric O-1 group of the glucose moiety at subsite 11, by corresponding segments from Ser426-Ala431 in trehalose phosphorylase and Thr419-Phe427 in kojibiose phosphorylase, thus confers LaMP with phosphorolytic activity towards trehalose and kojibiose, respectively. Substrate specificity of LaMP loop 3 variants in phosphorolysis, overview
additional information
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the (alpha/alpha)6-barrel loop 3 is a target for specificity engineering to achieve formation of trehalose and kojibiose by the enzyme in yields superior of maltose by reverse phosphorolysis with (alpha1, alpha1)- and alpha-(1,2)-regioselectivity, respectively. Substitution of LaMP His413-Glu421, His413-Ile418 and His413-Glu415 from loop 3, that include His413 and Glu415 presumably recognising the alpha-anomeric O-1 group of the glucose moiety at subsite 11, by corresponding segments from Ser426-Ala431 in trehalose phosphorylase and Thr419-Phe427 in kojibiose phosphorylase, thus confers LaMP with phosphorolytic activity towards trehalose and kojibiose, respectively. Substrate specificity of LaMP loop 3 variants in phosphorolysis, overview
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