Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

2.4.1.373: alpha-(1->2) branching sucrase

This is an abbreviated version!
For detailed information about alpha-(1->2) branching sucrase, go to the full flat file.

Reaction

sucrose
+
a (1->6)-alpha-D-glucan
=
D-fructose
 +
a (1->6)-alpha-D-glucan containing a (1->2)-alpha-D-glucose branch

Synonyms

brsA, dextransucrase, DsrE, GBD-CD2

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.373 alpha-(1->2) branching sucrase

Engineering

Engineering on EC 2.4.1.373 - alpha-(1->2) branching sucrase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
W2135F
mutant displays 3-4fold improved activity with naringenin
W2135G
W2135I/F2136Y
mutant displays 3-4fold improved activity with naringenin, about 60% conversion. Mutant converts nearly 3-times more naringenin than luteolin (59% and 17%, conversion, respectively)
W2135L-F2136L
mutant significantly synthesizes quercetin di-glucoside
W2135V
mutant displays 3-4fold improved activity with naringenin
additional information
expression of truncated forms of enzyme containing either catalytic domain CD1 or CD2. Enzymes devoid of CD2 synthesize products containing only alpha-1,6 linkages. Enzymes devoid of CD1 modified alpha-1,6 linear oligosaccharides and dextran acceptors through the formation of alpha-1,2 linkages