2.4.1.216: trehalose 6-phosphate phosphorylase

This is an abbreviated version!
For detailed information about trehalose 6-phosphate phosphorylase, go to the full flat file.

Word Map on EC 2.4.1.216

2.4.1.216

inguinal

hernia

mesh

sheath

preperitoneal

lichtenstein

postoperative

lactis

1-phosphate

phosphorolysis

maltose

posterior

department

unilateral

outpatient

endoscopic

files

rectus

expertise

enterococcus

beta-phosphoglucomutase

ethic

beta-glucose

netherlands

groin

high-volume

phosphorylases

re-recurrence

expert

elective

faecalis

synthesis

Reaction

Synonyms

TPP, trehalose-6-phosphate phosphatase, TrePP

ECTree

2 Transferases

2.4 Glycosyltransferases

2.4.1 Hexosyltransferases

2.4.1.216 trehalose 6-phosphate phosphorylase

Advanced search results

Do not include text mining results

Include

results (more...)

Include

results (more...)

Results	in table
94	AA Sequence
1	Application
1	CAS Registry Number
4	Cloned(Commentary)
2	General Information
1	Inhibitors
1	Ki Value [mM]
6	KM Value [mM]
4	Molecular Weight [Da]
30	Natural Substrates/ Products (Substrates)
37	Organism
1	Pathway
3	pH Optimum
2	pH Stability
2	Protein Variants
2	Purification (Commentary)
1	Reaction
3	Reaction Type
34	Reference
30	Source Tissue
3	Specific Activity [micromol/min/mg]
1	Storage Stability
37	Substrates and Products (Substrate)
4	Subunits
9	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
1	Temperature Range [°C]
1	Temperature Stability [°C]
1	Turnover Number [1/s]

Application

print visible entries

print all entries

Application on EC 2.4.1.216 - trehalose 6-phosphate phosphorylase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide

Go to Application Search

synthesis

Lactococcus lactis subsp. lactis

efficient one-pot enzymatic synthesis of trehalose 6-phosphate. The one-pot reaction of trehalose 6-phosphate phosphorylase and maltose phosphorylase enables production of 65 mM trehalose 6-phosphate from 100 mM maltose, 100 mM Glc6P, and 20 mM inorganic phosphate. Addition of beta-phosphoglucomutase to this reaction produces glucose 6-phosphate from beta-glucose 1-phosphate and thus reduces requirement of glucose 6-phosphate as a starting material. Within the range of 20-469 mM inorganic phosphate tested, the 54 mM concentration yielded the highest amount of trehalose 6-phosphate (33 mM). Addition of yeast increases the yield because of its glucose consumption. From 100 mmol maltose and 60 mmol inorganic phosphate, the production of 37.5 mmol trehalose 6-phosphate in a one-pot reaction (100 ml) is achieved, and 9.4 g trehalose 6-phosphate dipotassium salt is obtained

759004