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2.4.1.186: glycogenin glucosyltransferase

This is an abbreviated version!
For detailed information about glycogenin glucosyltransferase, go to the full flat file.

Word Map on EC 2.4.1.186

Reaction

UDP-alpha-D-glucose
+
glycogenin
=
UDP
+
alpha-D-glucosylglycogenin

Synonyms

CeGN, CgGN, CgGN-alpha, CgGN-beta, CgGN-gamma, EC 2.4.1.112, glucosyltransferase, uridine diphosphoglucose-protein, glucosyltransferase, uridine diphosphoglucose-protein 4-alpha-, glycogen initiator synthase, glycogenin, glycogenin 1, glycogenin 2, glycogenin glycosyltransferase, glycogenin-1, glycogenin-2, glyogenin, GN-1, GN1, GN2, GNN, GYG, GYG1, GYG2, hGYG1, M-glycogenin, priming glucosyltransferase, proglycogen synthase, UDP-glucose protein transglucosylase, UDP-glucose-protein glucosyltransferase, UDP-glucose:protein glucosyltransferase, UDPGlc:protein transglucosylase, UPTG, uridine diphosphate glucose-protein transglucosylase I, uridine diphosphoglucose protein transglucosylase I, uridine diphosphoglucose-protein 4-alpha-glucosyltransferase, uridine diphosphoglucose-protein glucosyltransferase

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.186 glycogenin glucosyltransferase

Cloned

Cloned on EC 2.4.1.186 - glycogenin glucosyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cell-free expression of His-tagged wild-type and mutant enzymes
-
expressed in C2C12 cells
-
expressed in Escherichia coli strain Rosetta (DE3)
-
expression in COS cells
-
expression in Escherichia coli as a GST-tagged protein
expression of glucose-free apo-glycogenin in an Escherichia coli mutant lacking UDP-glucose, enzyme is active towards itself and other substrates
-
expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
expression of mutant Y194F in Escherichia coli
-
expression of wild-type and mutant enzymes in Saccharomyces cerevisiae
expression of wild-type gycogenin-1, its truncated mutant DELTA-270, and of glycogenin-1 point mutants in Escherichia coli strain CGSC 4997
expresssion of recombinant protein in the CGSC Escherichia coli cell line 4997, which lacks UDP-glucose pyrophosphorylase activity
-
functional expression of the wild-type enzyme in Escherichia coli
-
gene CGI_10023521, DNA and amino acid sequence determination and analysis, the Pacific oyster encodes three isozymes of glycogenin: CgGN-alpha, CgGN-beta, and CgGN-gamma, genetic structure and sequence comparisons, phylogenetic tree. The isolated three CgGN isoforms contain alternative exon regions. PCR-based quantitative expression analysis. Recombinant expression of FLAG-tagged enzymes in HEK-293T cells, recombinant expression of EGFP-tagged enzyme from pEGFP-N1-CgGN in HeLa cells
gene GYG-1, genotyping
gene GYG1, genotyping
gene GYG1, recombinant expression of functional GST-tagged human muscle glycogen synthase in complex with human glycogenin-1 in Spodoptera frugiperda Sf9 cells using a bicistronic pFastBac™-Dual expression vector and baculovirus transfection
gene GYG2, genotyping
isolation of cDNA
-
preparation of a truncated already glucosylated His-tag fusion protein in Escherichia coli ER2566, expression of the truncated non-glucosylated His-tag fusion protein in Escherichia coli CGSC 4997
recombinant expression of His6-tagged wild-type and mutant enzymes glycogenin-1 in HEK-293 cells. Coexpression of wild-type glycogenin-2 together with different glycogenin-1 variants using a cell-free system, coexpression of functional glycogenin-1 and glycogenin-2 increases the glucosylation of glycogenin-2
recombinant expression of His6-tagged wild-type and mutant glycogenin-2 in HEK-293 cells. Coexpression of wild-type glycogenin-2 together with different glycogenin-1 variants using a cell-free system, coexpression of functional glycogenin-1 and glycogenin-2 increases the glucosylation of glycogenin-2