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47.81
D-glucose
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pH 6.0, 30°C, recombinant enzyme
additional information
additional information
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2
sucrose
hydrolytic activity, at pH 6.0 and 37°C
2 - 3
sucrose
total activity, at pH 6.0 and 37°C
2.3
sucrose
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pH 6.6, mutant enzyme S173A
2.5
sucrose
mutant enzyme S164A, 0.5 U/ml of enzyme activity at 37°C and pH 6.0
3
sucrose
total activity, at pH 6.0 and 37°C
4
sucrose
hydrolytic activity, at pH 6.0 and 37°C
4.1
sucrose
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pH 6.6, mutant enzyme N252A
6.6
sucrose
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pH 6.6, wild-type enzyme
7.4
sucrose
mutant enzyme I341V, 0.5 U/ml of enzyme activity at 37°C and pH 6.0
8
sucrose
wild-type, 0.5 U/ml of enzyme activity at 37°C and pH 6.0
8
sucrose
total activity, at pH 6.0 and 37°C
8
sucrose
transfructosylating activity, at pH 6.0 and 37°C
9
sucrose
hydrolytic activity, at pH 6.0 and 37°C
9
sucrose
total activity, at pH 6.0 and 37°C
10.5
sucrose
mutant D309N
10.5
sucrose
mutant enzyme H243L, 0.5 U/ml of enzyme activity at 37°C and pH 6.0
11.4
sucrose
wild-type enzyme
11.9
sucrose
30°C, pH 5.0, wild-type enzyme
12.3
sucrose
30°C, pH 5.0, mutant enzyme D135N
13.1
sucrose
30°C, pH 5.0, mutant enzyme C395S
13.8
sucrose
30°C, pH 5.0, mutant enzyme C339S
17.1
sucrose
sucrose hydrolysis, pH 6.0-6.6, 60°C
18.5
sucrose
sucrose hydrolysis, pH 6.0-6.6, 60°C
19.6
sucrose
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wild type enzyme, hydrolytic activity, at pH 6.0 and 50°C
20
sucrose
hydrolytic activity, at pH 6.0 and 37°C
21
sucrose
hydrolytic activity, at pH 6.0 and 37°C
24.1
sucrose
sucrose hydrolysis, pH 6.0-6.6, 60°C
29.3
sucrose
mutant enzyme R433A, 0.5 U/ml of enzyme activity at 37°C and pH 6.0
30
sucrose
mutant enzyme R369K, 0.5 U/ml of enzyme activity at 37°C and pH 6.0
30
sucrose
total activity, at pH 6.0 and 37°C
31.9
sucrose
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pH 6.6, mutant enzyme W94A
33.6
sucrose
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at pH 6.5 and 37°C
36
sucrose
Q60114
25°C, pH 5.0, transfructosylation activity
40
sucrose
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sucrose hydrolysis, wild-type
40
sucrose
Q60114
25°C, pH 5.0, levan synthesis activity is monitored by following the increase in turbidity at 600 nm that occurrs due to the insoluble levan formation
44.46
sucrose
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wild type enzyme, total activity, at pH 6.0 and 50°C
44.74
sucrose
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mutant enzyme K372Y, hydrolytic activity, at pH 6.0 and 50°C
50.5
sucrose
at pH 6.5 and 37°C
51.9
sucrose
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pH 6.6, mutant enzyme Y421A
64
sucrose
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at pH 5.9 and 50°C
66.6
sucrose
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pH 6.6, mutant enzyme L118A
77.91
sucrose
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mutant enzyme K372Y, total activity, at pH 6.0 and 50°C
86.69
sucrose
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mutant enzyme K372A, hydrolytic activity, at pH 6.0 and 50°C
110
sucrose
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sucrose hydrolysis, mutant R331K
113.13
sucrose
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mutant enzyme K372A, transfructosylation activity, at pH 6.0 and 50°C
117.2
sucrose
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pH 6.5, 40°C
118
sucrose
mutant enzyme E404K, at pH 6.5 and 45°C
120
sucrose
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sucrose hydrolysis, mutant R331H
125
sucrose
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wild-type enzyme
127.96
sucrose
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mutant enzyme N251W, transfructosylation activity, at pH 6.0 and 50°C
127.96
sucrose
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wild type enzyme, transfructosylation activity, at pH 6.0 and 50°C
131
sucrose
at pH 6.0 and 37°C
137.62
sucrose
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mutant enzyme N251W/K372Y, transfructosylation activity, at pH 6.0 and 50°C
154
sucrose
mutant enzyme R360S, 0.5 U/ml of enzyme activity at 37°C and pH 6.0
160
sucrose
sucrose hydrolysis, pH 5.8-6.6, 60°C
176.72
sucrose
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mutant enzyme K372Y, transfructosylation activity, at pH 6.0 and 50°C
180
sucrose
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sucrose hydrolysis, mutants R331L and R331S
193.4
sucrose
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mutant enzyme K372A, total activity, at pH 6.0 and 50°C
249
sucrose
mutant enzyme E404P, at pH 6.5 and 45°C
273
sucrose
mutant enzyme E404M, at pH 6.5 and 45°C
283
sucrose
mutant enzyme E404Q, at pH 6.5 and 45°C
284
sucrose
mutant enzyme E404F, at pH 6.5 and 45°C
286
sucrose
mutant enzyme E404H, at pH 6.5 and 45°C
287
sucrose
mutant enzyme E404Y, at pH 6.5 and 45°C
293
sucrose
mutant enzyme E404G, at pH 6.5 and 45°C
294
sucrose
mutant enzyme E404W, at pH 6.5 and 45°C
296
sucrose
mutant enzyme E404L, at pH 6.5 and 45°C
296
sucrose
mutant enzyme E404N, at pH 6.5 and 45°C
296
sucrose
mutant enzyme E404S, at pH 6.5 and 45°C
297.3
sucrose
mutant enzyme G361F, 0.5 U/ml of enzyme activity at 37°C and pH 6.0
305
sucrose
wild type enzyme, at pH 6.5 and 45°C
309
sucrose
mutant enzyme E404V, at pH 6.5 and 45°C
318
sucrose
mutant enzyme E404I, at pH 6.5 and 45°C
319.4
sucrose
mutant enzyme Y429N, 0.5 U/ml of enzyme activity at 37°C and pH 6.0
338
sucrose
mutant enzyme E404T, at pH 6.5 and 45°C
346
sucrose
mutant enzyme E404D, at pH 6.5 and 45°C
355
sucrose
mutant enzyme E404A, at pH 6.5 and 45°C
370
sucrose
mutant enzyme E404C, at pH 6.5 and 45°C
377
sucrose
mutant enzyme E404R, at pH 6.5 and 45°C
436
sucrose
transfructosylating activity, at pH 6.0 and 37°C
440
sucrose
transfructosylating activity, at pH 6.0 and 37°C
467
sucrose
transfructosylating activity, at pH 6.0 and 37°C
479
sucrose
transfructosylating activity, at pH 6.0 and 37°C
480
sucrose
total activity, at pH 6.0 and 37°C
480.4
sucrose
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pH 6.6, mutant enzyme W172A
519
sucrose
transfructosylating activity, at pH 6.0 and 37°C
617
sucrose
hydrolytic activity, at pH 6.0 and 37°C
674
sucrose
transfructosylating activity, at pH 6.0 and 37°C
795.22
sucrose
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mutant enzyme N251W/K372Y, total activity, at pH 6.0 and 50°C
820.75
sucrose
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mutant enzyme N251W, hydrolytic activity, at pH 6.0 and 50°C
978.37
sucrose
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mutant enzyme N251W, total activity, at pH 6.0 and 50°C
additional information
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additional information
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mutants, overview
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additional information
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Michaelis-Menten kinetics
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additional information
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Michaelis-Menten kinetics
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additional information
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Michaelis-Menten kinetics
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kinetics, overview
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additional information
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2.3 mM (sucrose) total, mutant enzyme S173A
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additional information
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29.2 mM (sucrose) total, mutant enzyme R370A, a 4fold KM value decrease compared with the wild-type enzyme supported the interactions of Arg 370 with the 2-OH and 3-OH of the glucosyl residue in the active site
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additional information
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31.9 mM (sucrose) total, mutant enzyme W94A, 9% of the catalytic efficiency of the wild-type
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additional information
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Q60114
32 mM (sucrose), 25°C, pH 5.0, total activity
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additional information
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32 mM (sucrose), 25°C, pH 5.0, total activity
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additional information
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35.1 mM (sucrose) total, mutant enzyme N252H
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additional information
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4.1 mM (sucrose) total, mutant enzyme N252A
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additional information
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480.4 mM (sucrose) total, mutant enzyme W172A, 72fold increase of the KM of the wild-type
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additional information
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51.9 mM (sucrose) total, mutant enzyme Y421A, 3%of the wild-type activity
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additional information
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6.6 mM (sucrose) total, wild-type, pH 6.6, 37°C, 50 mM Sorensens phosphate buffer, 7.36 mg/l enzyme concentration, reaction time 60 min
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66.6 mM (sucrose), mutant enzyme L118A
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7.5 mM (sucrose) total, mutant enzyme N252G
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8.4 mM (sucrose) total, mutant enzyme N252D
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Q60114
at pHs higher than 7.0, Zymomonas mobilis levansucrase does not exhibit levan synthesis activity
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additional information
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at pHs higher than 7.0, Zymomonas mobilis levansucrase does not exhibit levan synthesis activity
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mutant enzyme D257A, no activity
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additional information
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mutant enzyme D95A, no activity
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mutant enzyme E350A, nearly inactive
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mutant enzyme E352A, no measureable activity
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mutant enzyme R256A, nearly inactive
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additional information
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mutant enzyme S164K is inactive
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additional information
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mutant enzyme S164K is inactive
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additional information
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mutants A344P and F414W have the same behavior like the wild-type
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additional information
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mutants A344P and F414W have the same behavior like the wild-type
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additional information
additional information
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kinetics of free and immobilized enzymes, overview
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