2.3.1.268: ethanol O-acetyltransferase
This is an abbreviated version!
For detailed information about ethanol O-acetyltransferase, go to the full flat file.
Reaction
Synonyms
alcohol acetyltransferase, eat1, ethanol acetyltransferase, ethanol acetyltransferase 1, Wanomala_5543
ECTree
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Engineering
Engineering on EC 2.3.1.268 - ethanol O-acetyltransferase
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D145A
mutation in Ser-Asp-His catalytic triad, loss of activity
H295A
mutation in Ser-Asp-His catalytic triad, loss of activity
S121A
mutation in Ser-Asp-His catalytic triad, loss of activity
D145A
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mutation in Ser-Asp-His catalytic triad, loss of activity
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H295A
-
mutation in Ser-Asp-His catalytic triad, loss of activity
-
S121A
-
mutation in Ser-Asp-His catalytic triad, loss of activity
-
additional information
construction of an Eat1 knockout mutant and of N-terminally truncated mutants. Modulation of expression of the TCA cycle and electron transport chain genes using a highly multiplexed CRISPRi approach, simultaneous knockdown of ACO2b, SDH2, RIP1, and MSS51 results in a 3.8fold increase in ethyl acetate productivity over the already high natural capacity. Mitochondrial localization is partially lost when the first 14 amino acids are removed with complete cytosolic expression resulting from truncation at the predicted cut site, i.e. mutants Eat1(DELTA1-14) and Eat1(DELTA1-19)
additional information
-
construction of an Eat1 knockout mutant and of N-terminally truncated mutants. Modulation of expression of the TCA cycle and electron transport chain genes using a highly multiplexed CRISPRi approach, simultaneous knockdown of ACO2b, SDH2, RIP1, and MSS51 results in a 3.8fold increase in ethyl acetate productivity over the already high natural capacity. Mitochondrial localization is partially lost when the first 14 amino acids are removed with complete cytosolic expression resulting from truncation at the predicted cut site, i.e. mutants Eat1(DELTA1-14) and Eat1(DELTA1-19)
-
additional information
-
construction of an Eat1 knockout mutant and of N-terminally truncated mutants. Modulation of expression of the TCA cycle and electron transport chain genes using a highly multiplexed CRISPRi approach, simultaneous knockdown of ACO2b, SDH2, RIP1, and MSS51 results in a 3.8fold increase in ethyl acetate productivity over the already high natural capacity. Mitochondrial localization is partially lost when the first 14 amino acids are removed with complete cytosolic expression resulting from truncation at the predicted cut site, i.e. mutants Eat1(DELTA1-14) and Eat1(DELTA1-19)
-
additional information
-
construction of an Eat1 knockout mutant and of N-terminally truncated mutants. Modulation of expression of the TCA cycle and electron transport chain genes using a highly multiplexed CRISPRi approach, simultaneous knockdown of ACO2b, SDH2, RIP1, and MSS51 results in a 3.8fold increase in ethyl acetate productivity over the already high natural capacity. Mitochondrial localization is partially lost when the first 14 amino acids are removed with complete cytosolic expression resulting from truncation at the predicted cut site, i.e. mutants Eat1(DELTA1-14) and Eat1(DELTA1-19)
-
additional information
in Saccharomyces cerevisiae, the genes encoding phosphopantothenate-cysteine ligase, acetyl-CoA synthetase, and alcohol acetyltransferase are overexpressed by inserting the combined strong promoter PGK1p and the terminator PGK1t. The ethyl acetate levels of all engineered strains are enhanced. The final engineered strain CLy12a-ATF1-ACS2-CAB2 has an ethyl acetate yield of max. 610.26 mg/l, and the yield of higher alcohols is significantly decreased. The modification of ethyl acetate metabolic pathway is extremely important for the high level production of ethyl acetate in Saccharomyces cerevisiae
additional information
-
in Saccharomyces cerevisiae, the genes encoding phosphopantothenate-cysteine ligase, acetyl-CoA synthetase, and alcohol acetyltransferase are overexpressed by inserting the combined strong promoter PGK1p and the terminator PGK1t. The ethyl acetate levels of all engineered strains are enhanced. The final engineered strain CLy12a-ATF1-ACS2-CAB2 has an ethyl acetate yield of max. 610.26 mg/l, and the yield of higher alcohols is significantly decreased. The modification of ethyl acetate metabolic pathway is extremely important for the high level production of ethyl acetate in Saccharomyces cerevisiae
-