2.1.2.9: methionyl-tRNA formyltransferase

This is an abbreviated version!
For detailed information about methionyl-tRNA formyltransferase, go to the full flat file.

Reaction

Synonyms

FMT, FMT1, Fmt1p, formyl-methionyl transferase, formylmethionyl-transfer ribonucleic synthetase, methionyl ribonucleic formyltransferase, methionyl-transfer ribonucleic transformylase, methionyl-transfer RNA transformylase, methionyl-tRNA formyltransferase, methionyl-tRNA Met formyltransferase, methionyl-tRNA transformylase, methionyl-tRNA-Formyltransferase, methyltransferase, methionyl-transfer ribonucleate, mitochondrial methionyl-tRNA formyltransferase, MTF, MTFMT, N10-formyltetrahydrofolic-methionyl-transfer ribonucleic transformylase

ECTree

     2 Transferases

         2.1 Transferring one-carbon groups

             2.1.2 Hydroxymethyl-, formyl- and related transferases

                2.1.2.9 methionyl-tRNA formyltransferase

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Results	in table
2	Activating Compound
46946	AA Sequence
1	CAS Registry Number
14	Cloned(Commentary)
29	Cofactor
7	Crystallization (Commentary)
77	Disease/ Diagnostics
18	General Information
3	General Stability
17	Inhibitors
5	Ki Value [mM]
57	KM Value [mM]
27	Localization
27	Metals/Ions
11	Molecular Weight [Da]
44	Natural Substrates/ Products (Substrates)
2	Organic Solvent Stability
337	Organism
3	Pathway
18	PDB ID
5	pH Optimum
3	pH Range
71	Protein Variants
18	Purification (Commentary)
1	Reaction
1	Reaction Type
58	Reference
5	Source Tissue
12	Specific Activity [micromol/min/mg]
8	Storage Stability
239	Substrates and Products (Substrate)
5	Subunits
33	Synonyms
1	Systematic Name
16	Temperature Optimum [°C]
32	Turnover Number [1/s]

Purification

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Purification on EC 2.1.2.9 - methionyl-tRNA formyltransferase

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

3 entries

100-300fold, partial purification

Enterococcus faecalis

-

485802

10000fold purification

Escherichia coli

-

485797

1000fold purification from autotrophic cells, 650fold from chloroplasts

Euglena gracilis

-

485800

14fold purification of recombinant wild-type enzyme, overexpressed in Escherichia coli Novablue, purification of His-tagged G178E mutant enzyme

Escherichia coli

-

485810

affinity chromatography on Sepharose-bound tRNA

Escherichia coli

-

485795, 485797

over 1500fold purification

Escherichia coli

-

485794

partial

Triticum aestivum

-

485804

purification of His-tagged recombinant enzyme, expressed in Escherichia coli BL21(DE3)

Bos taurus

-

485807, 485808

purification of His-tagged wild-type and C-terminal deletion mutant recombinant enzymes, expressed in Escherichia coli

Escherichia coli

P23882

485812

purification of His6-tagged wild-type and mutant recombinant enzymes, expressed in Escherichia coli BL21(DE3)

Haemophilus influenzae

-

485814

purification of recombinant enzyme, expressed in Escherichia coli

Saccharomyces cerevisiae

-

485813

purification of wild-type and of R42K, R42L and G41R/R42L mutant recombinant enzymes, expressed in Escherichia coli JM109

Escherichia coli

-

485811

purification of wild-type enzyme and G41R, G41K mutant enzymes

Escherichia coli

-

485809

-

Escherichia coli

-

485802

-

Saccharomyces cerevisiae

-

485803

-

Escherichia coli

P23882

485815