2.1.1.240: trans-resveratrol di-O-methyltransferase
This is an abbreviated version!
For detailed information about trans-resveratrol di-O-methyltransferase, go to the full flat file.
Word Map on EC 2.1.1.240
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2.1.1.240
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stilbene
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pterostilbene
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vitis
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vinifera
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grape
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grapevine
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synthesis
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phytoalexins
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pinostilbene
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sorghum
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health-promoting
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bicolor
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piceatannol
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plasmopara
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rhubarb
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o-methylation
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viticola
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nutraceutical
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fungitoxic
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bioproduction
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riparia
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agroinfiltration
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peanut
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mono-methylated
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fungicidal
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berries
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di-methylated
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floral
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biotechnology
- 2.1.1.240
- stilbene
- pterostilbene
-
vitis
- vinifera
- grape
-
grapevine
- synthesis
-
phytoalexins
- pinostilbene
- sorghum
-
health-promoting
- bicolor
- piceatannol
-
plasmopara
- rhubarb
-
o-methylation
- viticola
-
nutraceutical
-
fungitoxic
-
bioproduction
- riparia
-
agroinfiltration
- peanut
-
mono-methylated
-
fungicidal
- berries
-
di-methylated
-
floral
- biotechnology
Reaction
2 S-adenosyl-L-methionine + = 2 S-adenosyl-L-homocysteine +
Synonyms
5-pentadecatrienyl resorcinol O-methyltransferase, MT, resveratrol O-methyltransferase, ROMT, SbOMT3, SbROMT3syn, SbROMT3syn protein, VrROMT, VrROMTsyn, VrROMTsyn protein, VvRO
ECTree
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Engineering
Engineering on EC 2.1.1.240 - trans-resveratrol di-O-methyltransferase
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additional information
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construction of a biological platform to produce pterostilbene with the ROMT gene of Arabidopsis thaliana. Pterostilbene can be synthesized from intracellular L-tyrosine, which requires the activities of four enzymes: tyrosine ammonia lyase (TAL) from Saccharothrix espanaensis, p-coumarate:CoA ligase (CCL) from Nicotiana tabacum, stilbene synthase (STS) from Vitis vinifera, and resveratrol O-methyltransferase (ROMT). For the efficient production of pterostilbene in Escherichia coli, an engineered Escherichia coli strain P1 is used to increase the intracellular pool of L-tyrosine, which is the initial precursor of pterostilbene, with L-mehionine containing media to increase the intracellular pool of S-adenosyl-L-methionine (SAM). Pterostilbene production as high as 33.6 mg/l is achieved, which is about 3.6fold higher compared with that in the parental Escherichia coli strain harboring a plasmid for pterostilbene biosynthesis. Method, overview
additional information
development of an Escherichia coli system containing an artificial biosynthetic pathway that produces methylated resveratrol analogues, such as pinostilbene (3,4'-dihydroxy-5-methoxystilbene), 3,5-dihydroxy-4'-methoxystilbene, 3,4'-dimethoxy-5-hydroxystilbene, and 3,5,4'-trimethoxystilbene, from simple carbon sources. The artificial biosynthetic pathways contain a series of codon-optimized O-methyltransferase genes from sorghum in addition to the resveratrol biosynthetic genes
additional information
metabolic engineering of stilbene biosynthesis, production of resveratrol and its monomethylated derivative pinostilbene as the major product from 4-coumaric acid in Escherichia coli through coexpression of multiple enzymes (cinnamate/4-coumarate:coenzyme A ligase, stilbene synthase, resveratrol O-methyltransferase) responsible for stilbene biosynthesis. When 4-coumaric acid is fed as the precursor, maximum levels of resveratrol and pinostilbene are produced by recombinant Escherichia coli cells co-expressing Streptomyces coelicolor cinnamate/4-coumarate:coenzyme A ligase ScCCL and Rheum palmatum stilbene synthase RpSTSsyn, or Streptomyces coelicolor ScCCL, RpSTSsyn and Sorghum bicolor resveratrol O-methyltransferase SbROMT3syn, respectively. Method evaluation and optimization
additional information
enhanced production of resveratrol derivatives in Nicotiana tabacum plants by improving the metabolic flux of intermediates in the phenylpropanoid pathway. Generation of transgenic Nicotiana tabacum SR1 plants (STS-OX and ROST-OX) expressing the RpSTS gene encoding stilbene synthase from rhubarb (Rheum palmatum cv. Jangyeop) and the RpSTS and VrROMT genes encoding resveratrol O-methyltransferase from frost grape (Vitis riparia) under the control of 35S promoter. Phenotypes and method development and evaluation, overview
additional information
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enhanced production of resveratrol derivatives in Nicotiana tabacum plants by improving the metabolic flux of intermediates in the phenylpropanoid pathway. Generation of transgenic Nicotiana tabacum SR1 plants (STS-OX and ROST-OX) expressing the RpSTS gene encoding stilbene synthase from rhubarb (Rheum palmatum cv. Jangyeop) and the RpSTS and VrROMT genes encoding resveratrol O-methyltransferase from frost grape (Vitis riparia) under the control of 35S promoter. Phenotypes and method development and evaluation, overview
additional information
functional expression of plant-derived O-methyltransferase, flavanone 3-hydroxylase, and flavonol synthase in Corynebacterium glutamicum and in Vitis vinifera cell culture for production of pterostilbene, kaempferol, and quercetin