1.14.99.52: L-cysteinyl-L-histidinylsulfoxide synthase
This is an abbreviated version!
For detailed information about L-cysteinyl-L-histidinylsulfoxide synthase, go to the full flat file.
Reaction
Synonyms
5-histidylcysteine sulfoxide synthase, OvoA, OvoA-like protein, OvoAe, OvoAErwin, OvoAt, OvoA_1, OvoA_2, PlOvoA, short OvoA homologue, SpOvoA, sulfoxide synthase
ECTree
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Engineering
Engineering on EC 1.14.99.52 - L-cysteinyl-L-histidinylsulfoxide synthase
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E176A
site-directed mutagenesis, the mutant shows reduced enzyme activity by at least 100fold compared to the wild-type enzyme
E176H
H170A
site-directed mutagenesis, the mutant shows reduced enzyme activity by at least 100fold compared to the wild-type enzyme
H174A
site-directed mutagenesis, the mutant shows reduced enzyme activity by at least 100fold compared to the wild-type enzyme
E176A
-
site-directed mutagenesis, the mutant shows reduced enzyme activity by at least 100fold compared to the wild-type enzyme
-
H170A
-
site-directed mutagenesis, the mutant shows reduced enzyme activity by at least 100fold compared to the wild-type enzyme
-
H174A
-
site-directed mutagenesis, the mutant shows reduced enzyme activity by at least 100fold compared to the wild-type enzyme
-
E176H
-
site-directed mutagenesis, the mutation changes OvoA from a 2-His-1-carboxylate to a 3-His ligand environment if the new third histidine residue replaces glutamate as the metal ligand. The mutant enzyme not only loses the cysteine dioxygenase activity but also stops catalyzing the formation of oxidative coupling products S-(L-histidin-5-yl)-L-cysteine S-oxide and cysteine sulfinic acid. The 2-His-1-carboxylate catalytic triad disrupts the cysteine dioxygenase activity